唾液微生物多样性--印度东部多囊卵巢综合征可能的生物标志物研究

Kusum Ghosh, Shreyoshi Chakraborty, D. Chatterjee, A. Bandyopadhyay
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引用次数: 0

摘要

导言和目的。多囊卵巢综合征(PCOS)是主要的生殖健康问题之一,被认为是多因素造成的,由于其发病率在过去几年中迅速上升(30%),作为主要给印度等发展中国家造成的双重负担(健康和经济),需要引起高度重视。因此,对这种疾病进行广泛和自由的筛查,以便进行预后、诊断和干预,似乎是一个紧迫的研究领域。在此背景下,本研究试图揭示唾液微生物多样性与多囊卵巢综合症的关系。材料和方法。为了达到研究目的,研究人员考虑了 100 名临床诊断为多囊卵巢综合症的患者和 110 名年龄匹配的非多囊卵巢综合症患者,他们都来自印度西孟加拉邦的孟加拉印度种姓人群。获得的唾液样本经 16S rDNA 扩增鉴定,微生物多样性经 Alu I 限制性酶消化测定。结果本研究显示,与非多囊卵巢综合征组相比,多囊卵巢综合征患者的 DNA 片段长度在 200 bp 和 225 bp 之间。结论。作为印度的首次尝试,本研究的主要特点是将唾液微生物多样性作为多囊卵巢综合征的另一种潜在和经济的生物标志物,从而激发 21 世纪人类学研究的新视野--微生物人类学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Salivary microbial diversity – an investigation on possible biomarkers for polycystic ovarian syndrome from eastern India
Introduction and aim. Polycystic ovarian syndrome (PCOS) is one of the major reproductive health issues, thought to be multifactorial, needs serious attention as a dual burden (health and economic) mainly for developing countries like India, due to its rapid rise (30%) in the last couple of years. Therefore, widespread and liberal screening for this disorder towards prognosis, diagnosis and intervention seems to be an urgent area of research. In this background, the present study attempts to unravel the association of salivary microbial diversity and PCOS. Material and methods. To achieve the purpose 100 clinically diagnosed PCOS individuals and 110 age matched non-PCOS participants from Bengalee Hindu caste population, West Bengal, India was considered. Obtained salivary samples were identified with 16S rDNA amplification and microbial diversity were determined by Alu I restriction enzyme digestion. Results. The present study revealed an explicit pattern of DNA fragment lengths varied between 200 bp and 225 bp in PCOs in comparison to the non-PCOS group. Conclusion. The cardinal feature of the present study as the first attempt from India envisaged, utilization of salivary microbial diversity as an additional potential and economizing biomarker for PCOS that stimulate new horizon of research in 21st century’s anthropology – the anthropology of microbes.
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