实验性溃疡性结肠炎结肠组织中前列腺素和环氧化酶的含量

I. Vasylyeva, Oksana A. Nakonechna, Liudmyla D. Popova, Olena V. Solomko, Kateryna В. Harbar, Natalia V. Yarmysh
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引用次数: 0

摘要

简介文章研究了 50%乙醇溶液中的 2,4-二硝基苯磺酸(DNBS)诱导的溃疡性结肠炎患者结肠组织中前列腺素和环氧化酶(COX)含量的变化。根据所获得的结果,作者得出结论:除 PGI2 外,所研究参数含量的变化是由乙醇效应而非 DNBS 引起的。两种 COX 同工酶在正常结肠中均有表达,而在溃疡性结肠炎中则有所减少。 研究目的研究实验性溃疡性结肠炎结肠组织中的前列腺素(PGE2、PGI2、PGF2α、TBX2 和 8-异-PGF2α)以及 COX-1 和 -2 的含量。 材料与方法用酶免疫吸附法测定结肠组织中前列腺素和环氧化酶的含量,实验对象为三组性成熟的实验鼠,雌雄均为 WAG 群体(第一对照组--直肠内注射生理盐水;第二对照组--注射 50%乙醇;实验组--注射 50%乙醇中的 DNBS)。 结果实验组大鼠结肠组织中的 PGE2 和 PGI2 含量与对照 1 组和对照 2 组相比有显著性差异。与对照 1 组相比,对照 2 组中 PGE2 的含量也有所增加。对照 2 组与对照 1 组相比,PGI2 的增加并不明显。实验组和第二对照组的 TBX2 和 PGF2α 含量明显低于第一对照组。与两个对照组相比,实验组大鼠的 8-异-PGF2α(非酶促类固醇)含量明显更高。第二对照组的 8-异-PGF2α含量明显高于第一对照组。实验组和对照二组大鼠结肠组织中 COX 两种同工酶的含量均明显低于对照一组。 结论对照组大鼠结肠中 COX 两种同工酶均有表达,表明 COX-2 参与支持正常结肠组织的生理功能。除 PGI2 外,所有研究指标的变化均由乙醇而非 DNBS 引起。50% 乙醇和 50% 乙醇中的 DNBS 都会刺激脂质过氧化,8-iso-PGF2α 含量的显著增加证实了这一点。在实验性溃疡性结肠炎中 COX-1 和 COX-2 水平降低的背景下,PGE2 和 PGF2α 含量的变化很可能是一种旨在维持结肠平衡的适应性反应。PGI2 含量的变化是由 DNBS 而非乙醇引起的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
THE CONTENT OF PROSTANOIDS AND CYCLOOXYGENASES IN COLON TISSUE IN EXPERIMENTAL ULCERATIVE COLITIS
Introduction. The article examines changes in the content of prostaglandins and cyclooxygenases (COX) in colon tissue in ulcerative colitis induced by 2,4-dinitrobenzene sulfonic acid (DNBS) in a 50% ethanol solution. Based on the obtained results, the authors conclude that changes in the content of the studied parameters, except PGI2, are due to ethanol effect, not DNBS. Both COX isozymes are expressed in normal colon and reduced in ulcerative colitis. The aim. To study the prostanoids (PGE2, PGI2, PGF2α, TBX2 and 8-iso-PGF2α) and COX-1 and -2 contents in colon tissue in experimental ulcerative colitis. Materials and methods. The determination of prostanoids and cyclooxygenases contents in colon tissue by enzyme immunosorbent assay was carried out on three groups of sexually mature laboratory rats of both sexes of the WAG population (1st control group – intrarectal injection of saline; 2nd control group – injection of 50% ethanol; experimental group – injection of DNBS in 50% ethanol). Results. PGE2 and PGI2 contents in colon tissue of experimental group rats were statistically significantly higher compared 1st and 2nd control groups. The content of PGE2 was also increased in 2nd control group versus 1st control one. The increasing PGI2 in 2nd control group versus 1st control was not significant. TBX2 and PGF2α contents in experimental and 2nd control groups were significantly lower compared 1st control. 8-iso-PGF2α (non-enzymatically derived prostanoid) level in experimental group rats was significantly higher compared both controls. 8-iso-PGF2α content in 2nd control group was significantly higher compared 1st one. The content of both COX isoforms in colon tissue in experimental group and 2nd control group rats was significantly lower compared to 1st control group. Conclusions. Both isoforms of COX are expressed in control group colon indicating COX-2 involvement in supporting physiological functions of normal colon tissue. All studied indicators changes, except PGI2, are due to ethanol, not DNBS. Both 50% ethanol and DNBS in 50% ethanol stimulate lipid peroxidation, confirmed by significant increase in 8-iso-PGF2α content. PGE2 and PGF2α contents changes against the background of reduced levels of COX-1 and COX-2 in experimental ulcerative colitis are most likely an adaptive response aimed at maintaining colon homeostasis. PGI2 content changes are due to DNBS, and not to ethanol.
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