支持大豆生长的耐磷溶盐细菌(Glycine max (L.) Merr.)在盐碱土上的生长

Q3 Agricultural and Biological Sciences
Irina Smirnova, A. Sadanov, G. Baimakhanova, E. Faizulina, L. Tatarkina
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引用次数: 0

摘要

通讯作者:Irina SmirnovaIrina Smirnova Research and Production Center for Microbiology and Virology LLC, Almaty, Kazakhstan Email: iesmirnova@mail.ru 摘要:磷是影响作物产量的重要植物营养元素之一。通过使用能够刺激盐碱地上大豆生长的微生物接种剂来增加磷的供应量是一个很有前景的解决方案。然而,大多数磷酸盐溶解细菌不能在盐碱土上生长和调动磷酸盐。本研究的目的是从大豆根瘤中分离出耐盐性磷酸盐溶解细菌,并研究它们的耐盐性、磷酸盐溶解能力以及影响大豆在盐碱土中生长的能力。磷酸盐溶解分离菌来自大豆根圈。研究了它们在高盐分胁迫条件下的生长和磷酸盐动员能力,然后选出了五株菌株。桑格分子遗传方法鉴定表明,这些菌株分别是根瘤假单胞菌 FT2、韩国假单胞菌 FT4、假单胞菌 FM9、枯草芽孢杆菌 FM12 和芽孢杆菌 FC11。研究了高盐胁迫下植物激素吲哚-3-乙酸的产生情况,筛选出三株菌株(FT4、FM12 和 FC11),它们在盐胁迫下的吲哚-3-乙酸产生量略有下降,降幅为 20-22%。与未接种的对照变体相比,接种细菌可明显缓解盐胁迫,从大豆的生长指标可以看出:根的重量增加了 1.9-2.1 倍,大豆芽的重量增加了 2.0-2.2 倍,叶的重量增加了 2.5-3.5 倍,结节的数量增加了 1.7-2.0 倍。研究表明,接种磷酸盐溶性耐盐细菌可提高植物对盐胁迫的抵抗力,改善生长状况,促进大豆的生态适应。根据分离出的菌株,有可能为生长在盐碱地上的作物开发接种剂,这是一种很有前景的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phosphate-Solubilizing Salt-Tolerant Bacteria to Support Soybean Growth (Glycine max (L.) Merr.) On Saline Soils
Corresponding Author: Irina Smirnova Research and Production Center for Microbiology and Virology LLC, Almaty, Kazakhstan Email: iesmirnova@mail.ru Abstract: Phosphorus is one of the important elements of plant nutrition that affect crop productivity. Increasing the availability of phosphorus through the use of microbial inoculants capable of stimulating soybean growth on saline soils is a promising solution to the problem. However, most phosphatesolubilizing bacteria are not capable of the growth and mobilization of phosphates on saline soils. The purpose of this study was to isolate phosphate-solubilizing salt-tolerant bacteria from the soybean rhizosphere and to study their salt tolerance, phosphate solubilization, and ability to influence soybean growth in saline soils. Phosphate-solubilizing isolates were obtained from the soybean rhizosphere. Their ability to grow and mobilize phosphates under conditions of high salt stress was studied, after which five strains were selected. Identification by the Sanger molecular genetic method showed that the strains were Pseudomonas rhizosphaerae FT2, Ps. koreensis FT4, Ps. sp. FM9, Bacillus pumilus FM12, and B. sp. FC11. The production of the phytohormone indole-3-acetic acid under high salt stress was studied and three strains (FT4, FM12, and FC11) were selected in which the production of indole-3-acetic acid under salt stress decreased slightly by 20-22%. Inoculation with bacteria significantly mitigated salt stress, as evidenced by the growth indicators of soybeans; the weight of roots increased by 1.9-2.1 times, the weight of soybean shoots by 2.0-2.2 times, the weight of leaves by 2.5-3.5 times and the number of nodules by 1.7-2.0 times compared to the control variant without inoculation. The study showed that inoculation with phosphate-solubilizing salt-tolerant bacteria increased plant resistance to salt stress, improved growth, and promoted the ecological adaptation of soybeans. Based on the isolated strains, it is possible to develop inoculants for crops growing on saline soils, which is a promising strategy.
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来源期刊
OnLine Journal of Biological Sciences
OnLine Journal of Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
2.10
自引率
0.00%
发文量
35
期刊介绍: :: Cell biology :: Developmental biology :: Structural biology :: Microbiology :: Molecular biology & genetics :: Biochemistry :: Biotechnology :: Biodiversity :: Ecology :: Marine biology :: Plant biology :: Bioinformatics
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