两个 Escallonia( Escalloniales)物种的基因组组装草案。

IF 1.9 Q3 GENETICS & HEREDITY
Andre S Chanderbali, Christopher Dervinis, Ioana G Anghel, Douglas E Soltis, Pamela S Soltis, Felipe Zapata
{"title":"两个 Escallonia( Escalloniales)物种的基因组组装草案。","authors":"Andre S Chanderbali, Christopher Dervinis, Ioana G Anghel, Douglas E Soltis, Pamela S Soltis, Felipe Zapata","doi":"10.1186/s12863-023-01186-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Escallonia (Escalloniaceae) belongs to the Escalloniales, a diverse clade of flowering plants with unclear placement in the tree of life. Escallonia species show impressive morphological and ecological diversity and are widely distributed across three hotspots of biodiversity in the Neotropics. To shed light on the genomic substrate of this radiation and the phylogenetic placement of Escalloniales as well as to generate useful data for comparative evolutionary genomics across flowering plants, we produced and annotated draft genomes for two species of Escallonia.</p><p><strong>Data description: </strong>Genomic DNA from E. rubra and E. herrerae was sequenced with Oxford Nanopore sequencing chemistry, generating 3.4 and 12 million sequence reads with an average read length of 9.4 and 9.1 Kb (approximately 31 and 111 Gb of sequence data), respectively. In addition, we generated Illumina 100-bp paired-end short read data for E. rubra (approximately 75 Gb of sequence data). The Escallonia rubra genome was 566 Mb, with 3,233 contigs and an N50 of 285 Kb. The assembled genome for E. herrerae was 994 Mp, with 5,760 contigs and an N50 of 317 Kb. The genome sequences were annotated with 31,038 (E. rubra) and 47,905 (E. herrerea) protein-coding gene models supported by transcriptome/protein evidence and/or Pfam domain content. BUSCO assessments indicated completeness levels of approximately 98% for the genome assemblies and 88% for the genome annotations.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"1"},"PeriodicalIF":1.9000,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759652/pdf/","citationCount":"0","resultStr":"{\"title\":\"Draft genome assemblies for two species of Escallonia (Escalloniales).\",\"authors\":\"Andre S Chanderbali, Christopher Dervinis, Ioana G Anghel, Douglas E Soltis, Pamela S Soltis, Felipe Zapata\",\"doi\":\"10.1186/s12863-023-01186-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Escallonia (Escalloniaceae) belongs to the Escalloniales, a diverse clade of flowering plants with unclear placement in the tree of life. Escallonia species show impressive morphological and ecological diversity and are widely distributed across three hotspots of biodiversity in the Neotropics. To shed light on the genomic substrate of this radiation and the phylogenetic placement of Escalloniales as well as to generate useful data for comparative evolutionary genomics across flowering plants, we produced and annotated draft genomes for two species of Escallonia.</p><p><strong>Data description: </strong>Genomic DNA from E. rubra and E. herrerae was sequenced with Oxford Nanopore sequencing chemistry, generating 3.4 and 12 million sequence reads with an average read length of 9.4 and 9.1 Kb (approximately 31 and 111 Gb of sequence data), respectively. In addition, we generated Illumina 100-bp paired-end short read data for E. rubra (approximately 75 Gb of sequence data). The Escallonia rubra genome was 566 Mb, with 3,233 contigs and an N50 of 285 Kb. The assembled genome for E. herrerae was 994 Mp, with 5,760 contigs and an N50 of 317 Kb. The genome sequences were annotated with 31,038 (E. rubra) and 47,905 (E. herrerea) protein-coding gene models supported by transcriptome/protein evidence and/or Pfam domain content. BUSCO assessments indicated completeness levels of approximately 98% for the genome assemblies and 88% for the genome annotations.</p>\",\"PeriodicalId\":72427,\"journal\":{\"name\":\"BMC genomic data\",\"volume\":\"25 1\",\"pages\":\"1\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-01-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759652/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC genomic data\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s12863-023-01186-7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC genomic data","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s12863-023-01186-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

摘要

目的:Escallonia(Escalloniaceae)属于Escalloniales科,是开花植物中的一个多样性支系,在生命树中的位置尚不明确。Escallonia 物种表现出惊人的形态和生态多样性,广泛分布于新热带地区的三个生物多样性热点地区。为了揭示这种辐射的基因组基质和 Escalloniales 的系统发育位置,并为有花植物的比较进化基因组学提供有用的数据,我们制作并注释了两个 Escallonia 物种的基因组草案:rubra 和 E. herrerae 的基因组 DNA,分别产生了 340 万和 1200 万个序列读数,平均读数长度分别为 9.4 和 9.1 Kb(约 31 和 111 Gb 序列数据)。此外,我们还为红鬣蜥生成了 Illumina 100-bp 成对端短读数数据(约 75 Gb 序列数据)。Escallonia rubra 的基因组为 566 Mb,有 3,233 个等位基因,N50 为 285 Kb。E. herrerae 的组装基因组为 994 Mp,有 5,760 个等位基因,N50 为 317 Kb。基因组序列注释了 31,038 个(E. rubra)和 47,905 个(E. herrerea)有转录组/蛋白质证据和/或 Pfam 结构域内容支持的蛋白质编码基因模型。BUSCO 评估表明,基因组组装的完整性水平约为 98%,基因组注释的完整性水平约为 88%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Draft genome assemblies for two species of Escallonia (Escalloniales).

Objectives: Escallonia (Escalloniaceae) belongs to the Escalloniales, a diverse clade of flowering plants with unclear placement in the tree of life. Escallonia species show impressive morphological and ecological diversity and are widely distributed across three hotspots of biodiversity in the Neotropics. To shed light on the genomic substrate of this radiation and the phylogenetic placement of Escalloniales as well as to generate useful data for comparative evolutionary genomics across flowering plants, we produced and annotated draft genomes for two species of Escallonia.

Data description: Genomic DNA from E. rubra and E. herrerae was sequenced with Oxford Nanopore sequencing chemistry, generating 3.4 and 12 million sequence reads with an average read length of 9.4 and 9.1 Kb (approximately 31 and 111 Gb of sequence data), respectively. In addition, we generated Illumina 100-bp paired-end short read data for E. rubra (approximately 75 Gb of sequence data). The Escallonia rubra genome was 566 Mb, with 3,233 contigs and an N50 of 285 Kb. The assembled genome for E. herrerae was 994 Mp, with 5,760 contigs and an N50 of 317 Kb. The genome sequences were annotated with 31,038 (E. rubra) and 47,905 (E. herrerea) protein-coding gene models supported by transcriptome/protein evidence and/or Pfam domain content. BUSCO assessments indicated completeness levels of approximately 98% for the genome assemblies and 88% for the genome annotations.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.90
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信