{"title":"印度喀拉拉邦不同农业生态区域木薯粉虱（Bemisia tabaci (Gennadius)）的特征揭示了木薯害虫的不同生物型别","authors":"Harish E.R., Mani Chellappan, Deepu Mathew, T. Makesh Kumar, Ranjith M.T., Eldho Varghese, Henna M.K.","doi":"10.1007/s12600-023-01118-2","DOIUrl":null,"url":null,"abstract":"<p>Mosaic disease spread by the whitefly, <i>Bemisia tabaci</i>, is the major threat to cassava cultivation. This study reveals the variations in cassava whitefly populations across 13 agro-ecological zones of Kerala State, India, and their virulence. Discriminant analysis of morphometric measurements of whitefly pupa from different agro-ecological zones showed more evident distinction compared to adult, and analysis of the whitefly genome using 10 selected ISSR primers has revealed two major clusters within the Sulthan Bathery population, as out group. Sequence analysis of the mitochondrial cytochrome oxidase1 gene showed the presence of two whitefly biotypes, Asia I and Asia II 5. This is the first report of whitefly biotype Asia I infesting cassava. The genetic variation could be the result of isolated speciation under highly diverse elevations and agro-ecological conditions. Cassava plants infested with the Asia I biotype had very low cassava mosaic disease severity compared to those infested with the Asia II 5 biotype. The present study, for the first time, provides molecular evidence of the possibility of more than one biotype of <i>B. tabaci</i> infesting cassava in India.</p>","PeriodicalId":20220,"journal":{"name":"Phytoparasitica","volume":"498 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of cassava whitefly (Bemisia tabaci (Gennadius)) from diverse agro-ecological zones of Kerala, India, reveals the presence of different biotypes as pests in cassava\",\"authors\":\"Harish E.R., Mani Chellappan, Deepu Mathew, T. Makesh Kumar, Ranjith M.T., Eldho Varghese, Henna M.K.\",\"doi\":\"10.1007/s12600-023-01118-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Mosaic disease spread by the whitefly, <i>Bemisia tabaci</i>, is the major threat to cassava cultivation. This study reveals the variations in cassava whitefly populations across 13 agro-ecological zones of Kerala State, India, and their virulence. Discriminant analysis of morphometric measurements of whitefly pupa from different agro-ecological zones showed more evident distinction compared to adult, and analysis of the whitefly genome using 10 selected ISSR primers has revealed two major clusters within the Sulthan Bathery population, as out group. Sequence analysis of the mitochondrial cytochrome oxidase1 gene showed the presence of two whitefly biotypes, Asia I and Asia II 5. This is the first report of whitefly biotype Asia I infesting cassava. The genetic variation could be the result of isolated speciation under highly diverse elevations and agro-ecological conditions. Cassava plants infested with the Asia I biotype had very low cassava mosaic disease severity compared to those infested with the Asia II 5 biotype. The present study, for the first time, provides molecular evidence of the possibility of more than one biotype of <i>B. tabaci</i> infesting cassava in India.</p>\",\"PeriodicalId\":20220,\"journal\":{\"name\":\"Phytoparasitica\",\"volume\":\"498 1\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-12-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytoparasitica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12600-023-01118-2\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytoparasitica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12600-023-01118-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
木薯粉虱传播的马赛克病是木薯种植的主要威胁。这项研究揭示了印度喀拉拉邦 13 个农业生态区木薯粉虱种群的变化及其毒力。对来自不同农业生态区域的粉虱蛹的形态测量结果进行的判别分析显示，与成虫相比，粉虱的区别更为明显，而使用 10 种选定的 ISSR 引物对粉虱基因组进行的分析表明，在 Sulthan Bathery 群体中，有两个主要群集。对线粒体细胞色素氧化酶 1 基因的序列分析表明，存在两种粉虱生物型，即亚洲 I 型和亚洲 II 5 型。 这是首次报告粉虱生物型亚洲 I 型侵染木薯。这种遗传变异可能是在高度不同的海拔和农业生态条件下孤立物种分化的结果。受亚洲 I 型生物型侵染的木薯植株与受亚洲 II 5 型生物型侵染的植株相比，木薯马赛克病的严重程度很低。本研究首次提供了分子证据，证明在印度木薯中可能存在不止一种 B. tabaci 生物型。
Characterization of cassava whitefly (Bemisia tabaci (Gennadius)) from diverse agro-ecological zones of Kerala, India, reveals the presence of different biotypes as pests in cassava
Mosaic disease spread by the whitefly, Bemisia tabaci, is the major threat to cassava cultivation. This study reveals the variations in cassava whitefly populations across 13 agro-ecological zones of Kerala State, India, and their virulence. Discriminant analysis of morphometric measurements of whitefly pupa from different agro-ecological zones showed more evident distinction compared to adult, and analysis of the whitefly genome using 10 selected ISSR primers has revealed two major clusters within the Sulthan Bathery population, as out group. Sequence analysis of the mitochondrial cytochrome oxidase1 gene showed the presence of two whitefly biotypes, Asia I and Asia II 5. This is the first report of whitefly biotype Asia I infesting cassava. The genetic variation could be the result of isolated speciation under highly diverse elevations and agro-ecological conditions. Cassava plants infested with the Asia I biotype had very low cassava mosaic disease severity compared to those infested with the Asia II 5 biotype. The present study, for the first time, provides molecular evidence of the possibility of more than one biotype of B. tabaci infesting cassava in India.
期刊介绍：
Phytoparasitica is an international journal on Plant Protection, that publishes original research contributions on the biological, chemical and molecular aspects of Entomology, Plant Pathology, Virology, Nematology, and Weed Sciences, which strives to improve scientific knowledge and technology for IPM, in forest and agroecosystems. Phytoparasitica emphasizes new insights into plant disease and pest etiology, epidemiology, host-parasite/pest biochemistry and cell biology, ecology and population biology, host genetics and resistance, disease vector biology, plant stress and biotic disorders, postharvest pathology and mycotoxins. Research can cover aspects related to the nature of plant diseases, pests and weeds, the causal agents, their spread, the losses they cause, crop loss assessment, and novel tactics and approaches for their management.
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