Chemical Fingerprint Analysis and Content Determination of Horned Gallnut and Bellied Gallnut in Galla Chinensis

To establish an objective and comprehensive methodology to analyse the connections and differences between horned gallnut (HG) and bellied gallnut (BG) in Galla Chinensis (GC). The HPLC fingerprints from 15 batches of HG and 15 batches of BG were performed, and chemometric analysis including similarity analysis (SA), hierarchical clustering analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares discrimination analysis (OPLS-DA) was also set up. The results showed that the similarity of all batch samples was more than 0.9. In fingerprint analysis, 8 distinct common peaks were detected, among which gallic acid (GA), 1,3,6-tri-O-galloyl-β-D-glucose (TGG), and 1,2,3,4,6-O-galloyl-D-glucose (PGG) were identified by comparing with the standard compounds. Meanwhile, samples were clearly grouped into two classifications corresponding to HG and BG. This study demonstrated that HPLC fingerprints coupled with chemometric analysis could be applied to discriminate HG and BG and evaluate the qualities of HG and BG rapidly, which provided a certain experimental basis for the selection of GC raw materials and subsequent use.