{"title":"乔治王岛菲尔德斯半岛南极冰冻杆菌 SO2 的冷适应和响应基因","authors":"","doi":"10.1007/s00300-023-03213-w","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p><em>Cryobacterium</em> spp. are Gram-positive bacteria that inhabit diverse geographical locations, particularly extremely cold environments like the Polar Regions. However, strategies that enable them to survive in harsh Antarctic environments are not fully understood. In this study, we conducted a comparative genomic analysis of the Antarctic <em>Cryobacterium</em> sp. SO2 as well as other members of the <em>Cryobacterium</em> genus. Phylogenetic analysis revealed that strain SO2 formed a distinct cluster with the validly described species: <em>C. adonitolivorans, C. actose, C. soli</em>, <em>C. arcticum</em>, and <em>C. zongtaii</em>. Comparative analysis based on ANI and AAI indicated that strain SO2 is a novel species. Relative Synonymous Codon Usage (RSCU) of <em>Cryobacterium</em> species exhibits a bias towards codon ending with G/C. The genomes of all strains harbored numerous genes associated with environmental-associated stress responses, including oxidative stress response, general stress response, heat-stress response, cold-stress response, cell envelope alteration, and osmotic stress response. Strain SO2 and related strains possess genes involved in breaking down and utilization of both plant and animal carbohydrate-containing materials. KEGG annotation indicated that strain SO2 and related species shared almost the same genes for the metabolism of trehalose (TreS, partial TPS/TPP, and TreY-TreZ pathway) and glycogen (classical and non-classical pathway). The results from this work helped us to better understand the genomic characteristics of <em>Cryobacterium</em> spp. in terms of genomic diversity and adaptation strategies, which may have significant implications for biotechnology and climate change research.</p>","PeriodicalId":20362,"journal":{"name":"Polar Biology","volume":"65 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cold adaptation and response genes of Antarctic Cryobacterium sp. SO2 from the Fildes Peninsula, King George Island\",\"authors\":\"\",\"doi\":\"10.1007/s00300-023-03213-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Abstract</h3> <p><em>Cryobacterium</em> spp. are Gram-positive bacteria that inhabit diverse geographical locations, particularly extremely cold environments like the Polar Regions. However, strategies that enable them to survive in harsh Antarctic environments are not fully understood. In this study, we conducted a comparative genomic analysis of the Antarctic <em>Cryobacterium</em> sp. SO2 as well as other members of the <em>Cryobacterium</em> genus. Phylogenetic analysis revealed that strain SO2 formed a distinct cluster with the validly described species: <em>C. adonitolivorans, C. actose, C. soli</em>, <em>C. arcticum</em>, and <em>C. zongtaii</em>. Comparative analysis based on ANI and AAI indicated that strain SO2 is a novel species. Relative Synonymous Codon Usage (RSCU) of <em>Cryobacterium</em> species exhibits a bias towards codon ending with G/C. The genomes of all strains harbored numerous genes associated with environmental-associated stress responses, including oxidative stress response, general stress response, heat-stress response, cold-stress response, cell envelope alteration, and osmotic stress response. Strain SO2 and related strains possess genes involved in breaking down and utilization of both plant and animal carbohydrate-containing materials. KEGG annotation indicated that strain SO2 and related species shared almost the same genes for the metabolism of trehalose (TreS, partial TPS/TPP, and TreY-TreZ pathway) and glycogen (classical and non-classical pathway). The results from this work helped us to better understand the genomic characteristics of <em>Cryobacterium</em> spp. in terms of genomic diversity and adaptation strategies, which may have significant implications for biotechnology and climate change research.</p>\",\"PeriodicalId\":20362,\"journal\":{\"name\":\"Polar Biology\",\"volume\":\"65 1\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-12-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polar Biology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s00300-023-03213-w\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIODIVERSITY CONSERVATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polar Biology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s00300-023-03213-w","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
Cold adaptation and response genes of Antarctic Cryobacterium sp. SO2 from the Fildes Peninsula, King George Island
Abstract
Cryobacterium spp. are Gram-positive bacteria that inhabit diverse geographical locations, particularly extremely cold environments like the Polar Regions. However, strategies that enable them to survive in harsh Antarctic environments are not fully understood. In this study, we conducted a comparative genomic analysis of the Antarctic Cryobacterium sp. SO2 as well as other members of the Cryobacterium genus. Phylogenetic analysis revealed that strain SO2 formed a distinct cluster with the validly described species: C. adonitolivorans, C. actose, C. soli, C. arcticum, and C. zongtaii. Comparative analysis based on ANI and AAI indicated that strain SO2 is a novel species. Relative Synonymous Codon Usage (RSCU) of Cryobacterium species exhibits a bias towards codon ending with G/C. The genomes of all strains harbored numerous genes associated with environmental-associated stress responses, including oxidative stress response, general stress response, heat-stress response, cold-stress response, cell envelope alteration, and osmotic stress response. Strain SO2 and related strains possess genes involved in breaking down and utilization of both plant and animal carbohydrate-containing materials. KEGG annotation indicated that strain SO2 and related species shared almost the same genes for the metabolism of trehalose (TreS, partial TPS/TPP, and TreY-TreZ pathway) and glycogen (classical and non-classical pathway). The results from this work helped us to better understand the genomic characteristics of Cryobacterium spp. in terms of genomic diversity and adaptation strategies, which may have significant implications for biotechnology and climate change research.
期刊介绍:
Polar Biology publishes Original Papers, Reviews, and Short Notes and is the focal point for biologists working in polar regions. It is also of interest to scientists working in biology in general, ecology and physiology, as well as in oceanography and climatology related to polar life. Polar Biology presents results of studies in plants, animals, and micro-organisms of marine, limnic and terrestrial habitats in polar and subpolar regions of both hemispheres.
Taxonomy/ Biogeography
Life History
Spatio-temporal Patterns in Abundance and Diversity
Ecological Interactions
Trophic Ecology
Ecophysiology/ Biochemistry of Adaptation
Biogeochemical Pathways and Cycles
Ecological Models
Human Impact/ Climate Change/ Conservation