豆类[即鹰嘴豆(Cicer arietinum)和扁豆(Lens culinaris)]蛋白提取物的酶改性对通心粉功能特性的影响"

Mohammed Saleh, Rania Ghanem, Youngseung Lee
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摘要

本研究探讨了酶水解鹰嘴豆和扁豆蛋白提取物对意大利面功能特性的影响。用 0、5、10、15 和 20% 的水解蛋白提取物以及扁豆和鹰嘴豆粉中的非水解提取物替代小麦粉。研究处理包括连续的酶解处理、酸处理和碱处理。豆类蛋白提取物的酶水解对豆类的功能特性有显著影响,无论采用哪种酶水解和/或化学提取方法,豆类蛋白水解量的增加都会导致吸水率的线性增加。结果表明,蛋白质水解后吸水率下降,而在蛋白质提取处理前进行酶水解,吸水率在各种处理中最高。此外,鹰嘴豆和扁豆处理对处理后的远红外图谱有影响,导致酶改性后的到达时间更快。例如,小扁豆的到达时间从 138% 缩短到 318%,鹰嘴豆的到达时间从 123% 缩短到 183%,这表明面团发育时间更快,有助于节省面食加工时间。在烹饪过程中,面食的烹饪损失从对照处理的 2.5%减少到酸碱处理鹰嘴豆的 1.2%(即减少 208%),以及处理扁豆样品的 0.4%(即减少 625%)。使用木瓜蛋白酶(EC 3.4.22.2)进行酶处理对减少熟面条的烹饪损失最有效。在对鹰嘴豆进行酸处理、连续酸处理和碱处理后,煮熟面条的硬度也从对照样本的 2010.6 N 分别降至 97.7、39.9 和 111.7 N。小扁豆也有类似的结果,硬度从 48.1、47.1 到 50.0 N 不等。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impacts of Enzymatic Modifications of Legume's [i.e., Chickpea (Cicer arietinum) and Lentil (Lens culinaris)] Protein Extracts on Pasta Functional Characteristics"
This study investigated the effect of enzymatic hydrolysis of chickpea and lentil protein extracts on pasta functional characteristics. Wheat flour was substituted with 0, 5, 10, 15,and 20% of hydrolyzed protein extract and non-hydrolyzed extracts from lentils and chickpea flours. Study treatment includes sequential enzymatic followed by acid and then alkaline treatments. Enzymatic hydrolysis of legume protein extracts had significant impacts on legume functional characteristics increase in legume protein hydrolysis resulted in a linear increase in water absorption regardless of enzymatic hydrolysis and/or chemical extraction method. Results showed a decrease in water absorption with protein hydrolysis while enzymatic hydrolysis before protein extraction treatment resulted in the greatest water absorption among treatments. Additionally, chickpea and lentil treatments impacted the treated farinograph resulting in faster arrival time with the enzymatic modification. For instance, arrival time decreased from 138 to 318% for lentils and from 123 to 183% for chickpea indicating a faster dough developing time that would contribute to saving in pasta processing time. Cooking loss of pasta during cooking decreased from 2.5% in the control treatment to 1.2% (i.e., 208% reduction) in the acid, alkaline treated chickpea and to 0.4% (i.e., 625% reduction) in treated lentil samples. The use of Papain (EC 3.4.22.2) enzymatic treatment was the most effective in decreasing the cooking loss of cooked pasta. Cooked pasta hardness also decreased from 2010.6 N in the control sample to 97.7, 39.9 and111.7 N for acidic, sequential acid then for the alkaline treatment of chickpea, respectively. Similar results were reported for lentils with hardness ranging from 48.1, 47.1 to 50.0 N. Therefore, the use of enzymatic modifications would contribute to improved pasta quality characteristics
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