马尾藻（特纳）硫酸化多糖（褐藻糖胶）的结构特征、抗氧化和抗病毒活性 J. Agardh

Q3 Chemistry

Chemistry Pub Date : 2023-12-12 DOI:10.3390/chemistry5040176

Abeer A. Ageeli, S. F. Mohamed

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引用次数: 0

摘要

褐藻含有多种酸性多糖，其中包括褐藻糖胶。本研究旨在对褐藻多糖进行提取和表征，以探索其抗病毒活性。研究人员从马尾藻粉末中提取了一种浅棕色硫酸化多糖（干重产率为 18%）。硫酸化多糖的分馏结果显示存在两种主要馏分：低硫酸化多糖（SPF1）和高硫酸化多糖（SPF2）。对 SPF2 进行的生物测定表明，与 SPF1 相比，SPF2 的抗氧化活性更高，120 分钟后的 IC50 值分别为 17 ± 1.3 µg/mL 和 31 ± 1.1 μg/mL。测定了 SPF2 对 Vero 细胞的细胞毒性，发现计算得出的半最大细胞毒性浓度（CC50）为 178 ± 1.05 µg/mL。根据这些结果，对 SPF2 进行了抗病毒活性检测。结果表明，与 1 型单纯疱疹病毒（HSV-1）相比，SPF2 对甲型肝炎病毒（HAV）具有更强的疗效，其相应的半最大抑制浓度（IC50）分别为 48 ± 1.8 µg/mL 和 123 ± 2.6 µg/mL。活性 SPF2 通过傅立叶变换红外光谱、1H 和 13C NMR 光谱进行表征。提取的褐藻糖胶可作为一种天然治疗剂，用于抗击各种病毒感染。

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Structural Characterization, Antioxidant, and Antiviral Activity of Sulfated Polysaccharide (Fucoidan) from Sargassum asperifolium (Turner) J. Agardh

Brown algae possess a diverse array of acidic polysaccharides, including fucoidan. The present research intends to investigate the extraction and characterization of algal polysaccharides to explore their antiviral activity. A light brown sulfated polysaccharide was extracted (with a yield of 18% of dry weight) from Sargassum asperifolium algal powder. The results of fractionation of sulfated polysaccharide revealed the occurrence of two primary fractions: low-sulfated polysaccharides (SPF1) and high-sulfated polysaccharides (SPF2). The bioassays conducted on SPF2 demonstrated a greater level of antioxidant activity compared to SPF1, with respective IC50 values of 17 ± 1.3 µg/mL and 31 ± 1.1 μg/mL after a duration of 120 min. The cytotoxicity of SPF2 on Vero cells was determined, and the calculated half-maximal cytotoxic concentration (CC50) was found to be 178 ± 1.05 µg/mL. Based on these results, an antiviral activity assay was conducted on SPF2. The results demonstrated that SPF2 had greater efficacy against Hepatitis A Virus (HAV) compared to Herpes Simplex Virus Type 1 (HSV-1), with corresponding half-maximal inhibitory concentrations (IC50) of 48 ± 1.8 µg/mL and 123 ± 2.6 µg/mL, respectively. The active SPF2 was characterized by FT-IR, 1H, and 13C NMR spectroscopy. The extracted fucoidan can be used as a natural therapeutic agent in combating various viral infections.

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来源期刊

Chemistry

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

11 weeks

期刊介绍： Chemistry—A European Journal is a truly international journal with top quality contributions (2017 ISI Impact Factor: 5.16). It publishes a wide range of outstanding Reviews, Minireviews, Concepts, Full Papers, and Communications from all areas of chemistry and related fields.