Wei Lu, Xiaoqiang Wang, Congyu Wang, Ke Gong, Jiawei Li, Xue Li, Peng Wang
{"title":"基于三电纳米发电机的自供电生物传感器,用于双特异性细菌检测","authors":"Wei Lu, Xiaoqiang Wang, Congyu Wang, Ke Gong, Jiawei Li, Xue Li, Peng Wang","doi":"10.1002/inf2.12508","DOIUrl":null,"url":null,"abstract":"<p>Pathogenic and corrosive bacteria pose a significant risk to human health or economic well-being. The specific, sensitive, and on-site detection of these bacteria is thus of paramount significance but remains challenging. Taking inspiration from immunoassays with primary and secondary antibodies, we describe here a rational design of microbial sensor (MS) under a dual-specificity recognition strategy using <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) as the detection model. In the MS, engineered aptamers are served as the primary recognition element, while polydopamine-<i>N</i>-acetyl-D-galactosamine (PDA-Gal NAc) nanoparticles are employed as the secondary recognition element, which will also generate and amplify changes in the output voltage signal. To achieve self-powering capability, the MS is constructed based on a triboelectric nanogenerator (TENG) with the specific aptamers immobilized on the TENG electrode surface. The as-prepared MS-TENG system exhibits good stability in output performance under external forces, and high specificity toward <i>P. aeruginosa</i>, with no cross-reactivity observed. A linear relationship (<i>R</i><sup>2</sup> = 0.995) between the output voltage and <i>P. aeruginosa</i> concentration is established, with a limit of detection estimated at around 8.7 × 10<sup>3</sup> CFU mL<sup>−1</sup>. The utilization of PDA-Gal NAc nanoparticles is found to play an important role in enhancing the specific and reliability of detection, and the underlying mechanisms are further clarified by computational simulations. In addition, the MS-TENG integrates a wireless communication module, enabling real-time monitoring of bacterial concentration on mobile devices. This work introduces a pioneering approach to designing self-powered smart microbial sensors with high specificity, using a double recognition strategy applicable to various bacteria beyond <i>P. aeruginosa</i>.\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":48538,"journal":{"name":"Infomat","volume":"6 3","pages":""},"PeriodicalIF":22.7000,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inf2.12508","citationCount":"0","resultStr":"{\"title\":\"A self-powered biosensor based on triboelectric nanogenerator for dual-specificity bacterial detection\",\"authors\":\"Wei Lu, Xiaoqiang Wang, Congyu Wang, Ke Gong, Jiawei Li, Xue Li, Peng Wang\",\"doi\":\"10.1002/inf2.12508\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Pathogenic and corrosive bacteria pose a significant risk to human health or economic well-being. The specific, sensitive, and on-site detection of these bacteria is thus of paramount significance but remains challenging. Taking inspiration from immunoassays with primary and secondary antibodies, we describe here a rational design of microbial sensor (MS) under a dual-specificity recognition strategy using <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) as the detection model. In the MS, engineered aptamers are served as the primary recognition element, while polydopamine-<i>N</i>-acetyl-D-galactosamine (PDA-Gal NAc) nanoparticles are employed as the secondary recognition element, which will also generate and amplify changes in the output voltage signal. To achieve self-powering capability, the MS is constructed based on a triboelectric nanogenerator (TENG) with the specific aptamers immobilized on the TENG electrode surface. The as-prepared MS-TENG system exhibits good stability in output performance under external forces, and high specificity toward <i>P. aeruginosa</i>, with no cross-reactivity observed. A linear relationship (<i>R</i><sup>2</sup> = 0.995) between the output voltage and <i>P. aeruginosa</i> concentration is established, with a limit of detection estimated at around 8.7 × 10<sup>3</sup> CFU mL<sup>−1</sup>. The utilization of PDA-Gal NAc nanoparticles is found to play an important role in enhancing the specific and reliability of detection, and the underlying mechanisms are further clarified by computational simulations. In addition, the MS-TENG integrates a wireless communication module, enabling real-time monitoring of bacterial concentration on mobile devices. 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A self-powered biosensor based on triboelectric nanogenerator for dual-specificity bacterial detection
Pathogenic and corrosive bacteria pose a significant risk to human health or economic well-being. The specific, sensitive, and on-site detection of these bacteria is thus of paramount significance but remains challenging. Taking inspiration from immunoassays with primary and secondary antibodies, we describe here a rational design of microbial sensor (MS) under a dual-specificity recognition strategy using Pseudomonas aeruginosa (P. aeruginosa) as the detection model. In the MS, engineered aptamers are served as the primary recognition element, while polydopamine-N-acetyl-D-galactosamine (PDA-Gal NAc) nanoparticles are employed as the secondary recognition element, which will also generate and amplify changes in the output voltage signal. To achieve self-powering capability, the MS is constructed based on a triboelectric nanogenerator (TENG) with the specific aptamers immobilized on the TENG electrode surface. The as-prepared MS-TENG system exhibits good stability in output performance under external forces, and high specificity toward P. aeruginosa, with no cross-reactivity observed. A linear relationship (R2 = 0.995) between the output voltage and P. aeruginosa concentration is established, with a limit of detection estimated at around 8.7 × 103 CFU mL−1. The utilization of PDA-Gal NAc nanoparticles is found to play an important role in enhancing the specific and reliability of detection, and the underlying mechanisms are further clarified by computational simulations. In addition, the MS-TENG integrates a wireless communication module, enabling real-time monitoring of bacterial concentration on mobile devices. This work introduces a pioneering approach to designing self-powered smart microbial sensors with high specificity, using a double recognition strategy applicable to various bacteria beyond P. aeruginosa.
期刊介绍:
InfoMat, an interdisciplinary and open-access journal, caters to the growing scientific interest in novel materials with unique electrical, optical, and magnetic properties, focusing on their applications in the rapid advancement of information technology. The journal serves as a high-quality platform for researchers across diverse scientific areas to share their findings, critical opinions, and foster collaboration between the materials science and information technology communities.