基于网络药理学和体外实验的肉苁蓉治疗心肌缺血再灌注(I/R)损伤的机制

IF 0.6 4区 医学 Q4 CHEMISTRY, MEDICINAL
Yuan-Jia Yue, Yu Li, Huimin Wang, Zhao Ji, Xing Rong, Lin Jiang
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引用次数: 0

摘要

通过网络药理学和细胞实验研究肉苁蓉(CDA)治疗心肌缺血再灌注(I/R)损伤的机制。CDA的主要活性成分来自中药系统药理学数据库和分析平台(TCMSP)。从DisGeNET、OMIMD和TTD中确定了I/R相关靶点,并利用STRING输入构建了I/R蛋白-蛋白相互作用(PPI)网络。对Matescape和Microshengxin中CDA抑制I/R损伤的靶点进行了基因本体(GO)分析和京都基因组百科全书(KEGG)通路富集分析。测定了细胞活力、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的水平,以及磷脂酰肌醇3-激酶(PI3K)和丝氨酸/苏氨酸激酶1(Akt1)的蛋白表达。共鉴定出236个靶点,其中PI3K、Akt、上皮生长因子受体(EGFR)和另一种激酶是主要靶点,根据GO和KEGG分析,CDA最有可能通过PI3K-Akt途径抑制I/R。10%的CDA药用血清浓度被认为是最有效的浓度。CDA组和BEZ23组的LDH和MDA水平明显下降,但SOD水平明显上升,从而减轻了细胞损伤。此外,CDA组中PI3K、Akt和p-AKT蛋白的表达明显减少。CDA通过抗氧化和抑制PI3K/Akt信号通路减轻了I/R损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanism of Cistanche deserticola Ma in the Treatment of Myocardial Ischemia-Reperfusion (I/R) Injury Based on Network Pharmacology and In Vitro Experiments
To investigate the mechanism of Cistanche deserticola Ma (CDA) in the treatment of myocardial ischemia-reperfusion (I/R) injury by network pharmacology and cell experiments. The main active components of CDA were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). I/R-related targets were identified from DisGeNET, OMIMD, and TTD; the I/R protein–protein interaction (PPI) network was constructed using the STRING input. The targets of CDA that inhibit I/R injury in Matescape and Microshengxin were subjected to Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Cell viability, levels of lactate dehydrogenase (LDH), superoxide dismutase (SOD), and malondialdehyde (MDA), and protein expression of phosphatidylinositol 3-kinase (PI3K) and serine/threonine kinase 1 (Akt1) were determined. A total of 236 targets were identified, with PI3K, Akt, epithelial growth factor receptor (EGFR), and another kinase being the major targets, and according to GO and KEGG analysis, CDA was most likely to inhibit I/R through the PI3K-Akt pathway. The optimal concentration of 10% medicated serum of CDA was determined to be the most effective concentration. The levels of LDH and MDA were significantly decreased in the CDA and BEZ23 groups, but the levels of SOD were significantly increased, thereby alleviating cell damage. In addition, the expression of PI3K, Akt, and p-AKT proteins was significantly reduced in the CDA group. CDA alleviates I/R injury through antioxidation and inhibition of the PI3K/Akt signaling pathway.
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来源期刊
Pharmacognosy Magazine
Pharmacognosy Magazine CHEMISTRY, MEDICINAL-
CiteScore
1.87
自引率
0.00%
发文量
37
审稿时长
3 months
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