藏羊钙蛋白基因的单核苷酸多态性及其与生长性状的关系

IF 1.2 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Zhanhong Gao, S. A. Raza, Boyan Ma, Fengshuo Zhang, Shengzhen Hou, Hessah Alshammar, Essam Eldin Abdelhady Salama, W. Abdulmonem, Y. M. Alharbi, Abdullah S. M. Aljohani, A. El-Mansi, A. A. Abd El-Aziz, B. Aloufi, Linsheng Gui
{"title":"藏羊钙蛋白基因的单核苷酸多态性及其与生长性状的关系","authors":"Zhanhong Gao, S. A. Raza, Boyan Ma, Fengshuo Zhang, Shengzhen Hou, Hessah Alshammar, Essam Eldin Abdelhady Salama, W. Abdulmonem, Y. M. Alharbi, Abdullah S. M. Aljohani, A. El-Mansi, A. A. Abd El-Aziz, B. Aloufi, Linsheng Gui","doi":"10.1139/cjas-2023-0102","DOIUrl":null,"url":null,"abstract":"The calpastatin gene has been extensively studied as a candidate gene because of its regulatory function within muscle development of animals. However, little is known about the association between variation of the calpastatin gene and growth traits in Tibetan sheep. Using DNA sequencing, four single nucleotide polymorphisms (SNPs) g.3844A>G, g.88874T>C, g.89126C>T and g.89157A>C were identified in this study. An association analysis indicates that the g.3844A>G and g.89126C>T polymorphisms affected body weight (P<0.05). The g.89157A>C polymorphism was a significantly correlated with body weight and chest circumference (P<0.05). The quantitative real-time polymerase chain reaction (qPCR) analysis revealed that the expression of calpastatin gene presented an increasing trend with an increase in age. Remarkably lower mRNA expression was detected at the fetal stage compared with adult ewes (P<0.05). These findings indicated that the calpastatin gene polymorphisms were involved in growth-related traits in Tibetan sheep, which can be considered as genetic markers for improving the growth traits of Chinese Tibetan sheep.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Single Nucleotide Polymorphisms in Calpastatin Gene and the Association with Growth Traits in Tibetan sheep (Ovis aries)\",\"authors\":\"Zhanhong Gao, S. A. Raza, Boyan Ma, Fengshuo Zhang, Shengzhen Hou, Hessah Alshammar, Essam Eldin Abdelhady Salama, W. Abdulmonem, Y. M. Alharbi, Abdullah S. M. Aljohani, A. El-Mansi, A. A. Abd El-Aziz, B. Aloufi, Linsheng Gui\",\"doi\":\"10.1139/cjas-2023-0102\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The calpastatin gene has been extensively studied as a candidate gene because of its regulatory function within muscle development of animals. However, little is known about the association between variation of the calpastatin gene and growth traits in Tibetan sheep. Using DNA sequencing, four single nucleotide polymorphisms (SNPs) g.3844A>G, g.88874T>C, g.89126C>T and g.89157A>C were identified in this study. An association analysis indicates that the g.3844A>G and g.89126C>T polymorphisms affected body weight (P<0.05). The g.89157A>C polymorphism was a significantly correlated with body weight and chest circumference (P<0.05). The quantitative real-time polymerase chain reaction (qPCR) analysis revealed that the expression of calpastatin gene presented an increasing trend with an increase in age. Remarkably lower mRNA expression was detected at the fetal stage compared with adult ewes (P<0.05). These findings indicated that the calpastatin gene polymorphisms were involved in growth-related traits in Tibetan sheep, which can be considered as genetic markers for improving the growth traits of Chinese Tibetan sheep.\",\"PeriodicalId\":9512,\"journal\":{\"name\":\"Canadian Journal of Animal Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-12-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian Journal of Animal Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1139/cjas-2023-0102\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Animal Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1139/cjas-2023-0102","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

由于钙磷脂基因在动物肌肉发育中的调控功能,它作为候选基因已被广泛研究。然而,人们对钙磷脂基因变异与藏羊生长性状之间的关系知之甚少。通过DNA测序,本研究发现了四个单核苷酸多态性(SNPs)g.3844A>G、g.88874T>C、g.89126C>T和g.89157A>C。关联分析表明,g.3844A>G 和 g.89126C>T 多态性会影响体重(PC 多态性与体重和胸围显著相关(P<0.05))。实时聚合酶链反应(qPCR)定量分析显示,随着年龄的增长,钙磷脂基因的表达呈上升趋势。与成年母羊相比,胎儿期母羊的 mRNA 表达量明显较低(P<0.05)。这些结果表明,钙蛋白基因多态性与藏羊的生长相关性状有关,可作为改善中国藏羊生长性状的遗传标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single Nucleotide Polymorphisms in Calpastatin Gene and the Association with Growth Traits in Tibetan sheep (Ovis aries)
The calpastatin gene has been extensively studied as a candidate gene because of its regulatory function within muscle development of animals. However, little is known about the association between variation of the calpastatin gene and growth traits in Tibetan sheep. Using DNA sequencing, four single nucleotide polymorphisms (SNPs) g.3844A>G, g.88874T>C, g.89126C>T and g.89157A>C were identified in this study. An association analysis indicates that the g.3844A>G and g.89126C>T polymorphisms affected body weight (P<0.05). The g.89157A>C polymorphism was a significantly correlated with body weight and chest circumference (P<0.05). The quantitative real-time polymerase chain reaction (qPCR) analysis revealed that the expression of calpastatin gene presented an increasing trend with an increase in age. Remarkably lower mRNA expression was detected at the fetal stage compared with adult ewes (P<0.05). These findings indicated that the calpastatin gene polymorphisms were involved in growth-related traits in Tibetan sheep, which can be considered as genetic markers for improving the growth traits of Chinese Tibetan sheep.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Canadian Journal of Animal Science
Canadian Journal of Animal Science 农林科学-奶制品与动物科学
CiteScore
2.30
自引率
0.00%
发文量
51
审稿时长
6 months
期刊介绍: Published since 1957, this quarterly journal contains new research on all aspects of animal agriculture and animal products, including breeding and genetics; cellular and molecular biology; growth and development; meat science; modelling animal systems; physiology and endocrinology; ruminant nutrition; non-ruminant nutrition; and welfare, behaviour, and management. It also publishes reviews, letters to the editor, abstracts of technical papers presented at the annual meeting of the Canadian Society of Animal Science, and occasionally conference proceedings.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信