易受伤害的拖鞋兰Cypripedium cordigerum D.的离体共生繁殖。唐

IF 0.6 Q4 ENVIRONMENTAL SCIENCES

European journal of environmental sciences Pub Date : 2023-12-17 DOI:10.14712/23361964.2023.10

S. Kaur

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引用次数: 0

摘要

本文试图利用体外共生种子萌发技术，为保护易危物种堇菜（Cypripedium cordigerum D. Don）制定一套方案。唐（Cypripedium cordigerum D.Don）的体外共生种子发芽法。使用不同的光周期（即每天 24 小时黑暗或 12 小时光照）测试了四种兰花种子萌发培养基[陆生兰花培养基（BM）、改良陆生兰花种子萌发培养基（BM-1）、Malmgren 改良陆生兰花培养基（MM）、Knudson C 培养基（KC）]的适宜性。在两个不同的发育阶段收获种子蒴果，以确定蒴果成熟度对种子萌发的影响。在 BM-1 培养基上，完整蒴果种子在黑暗条件下的最大发芽率为 65.00 ± 0.12%。在培养基中加入活性炭可阻止棕色渗出物的释放。幼苗发育时间为 26.17 ± 0.17 周。本研究首次报道了 C. cordigerum 的异地保护。

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In vitro asymbiotic propagation of the vulnerable slipper orchid Cypripedium cordigerum D. Don

This paper presents an attempt to establish a protocol for the conservation of Cypripedium cordigerum D. Don a vulnerable species of orchid using in vitro symbiotic seed germination. The suitability of four orchids seed germination media [Terrestrial orchid medium (BM), modified terrestrial orchid seed germination medium (BM-1), Malmgren modified terrestrial orchid medium (MM), Knudson C medium (KC)] was tested using different photoperiods i.e. 24-h dark or 12-h light per day. The seed capsules were harvested at two different stages of development in order to determine the effect of capsule maturity on seed germination. The maximum percentage germination of seed from intact capsules in the dark was 65.00 ± 0.12% on the BM-1 medium. Release of brownish exudates was stopped by the addition of activated charcoal to the cultures. Seedlings developed in 26.17 ± 0.17 weeks. The current study is the first to report the ex-situ conservation of C. cordigerum.

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来源期刊

European journal of environmental sciences ENVIRONMENTAL SCIENCES-

CiteScore

1.50

自引率

12.50%

发文量

审稿时长

25 weeks

期刊介绍： The European Journal of Environmental Sciences offers a mixture of original refereed research papers, which bring you some of the most exciting developments in environmental sciences in the broadest sense, often with an inter- or trans-disciplinary perspective, focused on the European problems. The journal also includes critical reviews on topical issues, and overviews of the status of environmental protection in particular regions / countries. The journal covers a broad range of topics, including direct or indirect interactions between abiotic or biotic components of the environment, interactions of environment with human society, etc. The journal is published twice a year (June, December).