{"title":"从万年青养殖场分离出的具有硝化能力的本地卤单胞菌物种的多样性","authors":"Silvana Pérez Tejeda, Melissa Angélica Rojas Suárez, Gardenia Liszet Prado Chavarri, David Zavaleta-Verde","doi":"10.57188/manglar.2023.049","DOIUrl":null,"url":null,"abstract":"The objective of the research was to identify the species of the genus Halomonas, with nitrifying capacity, isolated from sediments of Litopenaeus vannamei farms from shrimp farms in Tumbes-Peru. From the sediment of 3 prawn farms at harvest time, a representative sample was obtained, to which a pre-enrichment was carried out in Marine Broth for 48 hours, then it was cultured in Marine Agar, until the observation of colonies characteristic of the genus Halomonas. The cultures obtained were identified phenotypically at the species level, to then evaluate their nitrifying capacity according to the Griess-llosvay methodology. As results, the isolation of 5 bacterial cultures was obtained, two identified as H. sulfidaeris and 3 as H. salina; All cultures showed the ability to reduce nitrate to nitrite by eliminating ammonium from the medium. It is concluded that in the sediment of Litopenaeus vannamei breeding sites located in Tumbes-Peru, the species H. sulfidaeris and H. salina are present, with nitrifying capacity, which allow the elimination of ammonium, which is developed as an effective option for the saline ammonium wastewater treatment.","PeriodicalId":34452,"journal":{"name":"Manglar","volume":"4 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diversidad de especies nativas de Halomonas con capacidad nitrificante aisladas de criaderos de Litopenaeus vannamei\",\"authors\":\"Silvana Pérez Tejeda, Melissa Angélica Rojas Suárez, Gardenia Liszet Prado Chavarri, David Zavaleta-Verde\",\"doi\":\"10.57188/manglar.2023.049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of the research was to identify the species of the genus Halomonas, with nitrifying capacity, isolated from sediments of Litopenaeus vannamei farms from shrimp farms in Tumbes-Peru. From the sediment of 3 prawn farms at harvest time, a representative sample was obtained, to which a pre-enrichment was carried out in Marine Broth for 48 hours, then it was cultured in Marine Agar, until the observation of colonies characteristic of the genus Halomonas. The cultures obtained were identified phenotypically at the species level, to then evaluate their nitrifying capacity according to the Griess-llosvay methodology. As results, the isolation of 5 bacterial cultures was obtained, two identified as H. sulfidaeris and 3 as H. salina; All cultures showed the ability to reduce nitrate to nitrite by eliminating ammonium from the medium. It is concluded that in the sediment of Litopenaeus vannamei breeding sites located in Tumbes-Peru, the species H. sulfidaeris and H. salina are present, with nitrifying capacity, which allow the elimination of ammonium, which is developed as an effective option for the saline ammonium wastewater treatment.\",\"PeriodicalId\":34452,\"journal\":{\"name\":\"Manglar\",\"volume\":\"4 2\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Manglar\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.57188/manglar.2023.049\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Manglar","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.57188/manglar.2023.049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究的目的是从秘鲁通贝斯(Tumbes-Peru)对虾养殖场的凡纳滨对虾(Litopenaeus vannamei)养殖场沉积物中分离出的具有硝化能力的卤单胞菌属(Halomonas)物种进行鉴定。从 3 个对虾养殖场收获时的沉积物中采集了具有代表性的样本,在海洋肉汤中进行 48 小时的预富集,然后在海洋琼脂中培养,直到观察到具有卤单胞菌属特征的菌落。对获得的培养物进行物种表型鉴定,然后根据 Griess-llosvay 方法评估其硝化能力。结果分离出 5 个细菌培养物,其中 2 个被鉴定为 H. sulfidaeris,3 个被鉴定为 H. salina;所有培养物均显示出通过消除培养基中的铵将硝酸盐还原为亚硝酸盐的能力。结论是,在位于通贝斯-秘鲁的凡纳滨对虾繁殖地的沉积物中,存在具有硝化能力的 H. sulfidaeris 和 H. salina 菌种,它们能够消除氨,是处理含盐氨废水的有效选择。
Diversidad de especies nativas de Halomonas con capacidad nitrificante aisladas de criaderos de Litopenaeus vannamei
The objective of the research was to identify the species of the genus Halomonas, with nitrifying capacity, isolated from sediments of Litopenaeus vannamei farms from shrimp farms in Tumbes-Peru. From the sediment of 3 prawn farms at harvest time, a representative sample was obtained, to which a pre-enrichment was carried out in Marine Broth for 48 hours, then it was cultured in Marine Agar, until the observation of colonies characteristic of the genus Halomonas. The cultures obtained were identified phenotypically at the species level, to then evaluate their nitrifying capacity according to the Griess-llosvay methodology. As results, the isolation of 5 bacterial cultures was obtained, two identified as H. sulfidaeris and 3 as H. salina; All cultures showed the ability to reduce nitrate to nitrite by eliminating ammonium from the medium. It is concluded that in the sediment of Litopenaeus vannamei breeding sites located in Tumbes-Peru, the species H. sulfidaeris and H. salina are present, with nitrifying capacity, which allow the elimination of ammonium, which is developed as an effective option for the saline ammonium wastewater treatment.