瓜子黄癣菌果体多糖的结构阐释和免疫活性研究

IF 2.5 4区工程技术 Q3 CHEMISTRY, ANALYTICAL

Separations Pub Date : 2023-12-20 DOI:10.3390/separations11010003

Qingqing Li, Ying Li, Huazhou Niu, Enhui Wang, Lili Jiao, Hui Li, Wei Wu

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引用次数: 0

摘要

多糖是黑木耳子实体的主要营养成分。多糖的结构和免疫活性具有重要的开发价值。本研究制备并测定了两种多糖，包括中性多糖（AMPN）和酸性多糖（AMPA）。采用离子交换柱和凝胶色谱柱对AMPN和AMPA进行分离和精制。分析表明，AMPN 和 AMPA 的分子量分别为 4.432 × 103 Da 和 7.323 × 103 Da。单糖成分分析表明，AMPN 主要由 68.3% 的葡萄糖组成，而 AMPA 主要由葡萄糖、甘露糖和半乳糖组成，此外还有 8.9% 的半乳糖醛酸和 3% 的岩藻糖。然后，通过红外光谱分析、刚果红染色、甲基化和核磁共振光谱分析等手段，明确了 AMPN 和 AMPA 的结构。核磁共振光谱显示，这两种多糖都含有α和β构型的糖苷键。用免疫缺陷小鼠比较了 AMPN 和 AMPA 对免疫修复能力的影响。活性实验表明，AMPN和从阿米巴子实体中提取的酸性多糖具有生物免疫活性。其中，AMPA 的免疫活性更高。这些研究结果表明，益母草子实体可作为膳食补充剂和功能性产品的来源。

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Structure Elucidation and Immunoactivity Study of Armillaria mellea Fruiting Body Polysaccharides

Polysaccharides are the main nutritional constituents in the Armillaria mellea fruiting bodies. The structure and immunoactivity of Armillaria mellea polysaccharide are valuable to be determined for development. In the present study, two polysaccharides, including Armillaria mellea neutral polysaccharide (AMPN) and Armillaria mellea acidic polysaccharide (AMPA), were prepared and determined. AMPN and AMPA were separated and refined by the ion exchange column and gel chromatographycolumn. Analysis of AMPN and AMPA revealed molecular weights of 4.432 × 103 Da and 7.323 × 103 Da. The monosaccharide composition analysis revealed that AMPN was mainly composed of 68.3%glucose, while AMPA consisted primarily of glucose, mannose, and galactose, accompanied by 8.9% galacturonic acid and 3% fucose. Then, infrared spectra analysis, Congo red staining, methylation, and NMR spectroscopy analysis were conducted as a means to clarify the structure of AMPN and AMPA. The NMR spectra demonstrated that the two polysaccharides included both α and β-configuration glycosidic bonds. The Congo red experiment suggests that AMPN and AMPA all had obvious triple helix structures.The effects of AMPN and AMPA on immune repair ability were compared by immune deficiency mice. The activity experiment showed that AMPN and acidic polysaccharides extracted from the Armillaria fruiting body have biological immune activity. Among them, AMPA showed higher immune activity. These findings suggest that Armillaria mellea fruiting bodies may be used as a source of dietary supplements and functional products.

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来源期刊

Separations Chemistry-Analytical Chemistry

CiteScore

3.00

自引率

15.40%

发文量

342

审稿时长

12 weeks

期刊介绍： Separations (formerly Chromatography, ISSN 2227-9075, CODEN: CHROBV) provides an advanced forum for separation and purification science and technology in all areas of chemical, biological and physical science. It publishes reviews, regular research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: Manuscripts regarding research proposals and research ideas will be particularly welcomed. Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Manuscripts concerning summaries and surveys on research cooperation and projects (that are funded by national governments) to give information for a broad field of users. The scope of the journal includes but is not limited to: Theory and methodology (theory of separation methods, sample preparation, instrumental and column developments, new separation methodologies, etc.) Equipment and techniques, novel hyphenated analytical solutions (significantly extended by their combination with spectroscopic methods and in particular, mass spectrometry) Novel analysis approaches and applications to solve analytical challenges which utilize chromatographic separations as a key step in the overall solution Computational modelling of separations for the purpose of fundamental understanding and/or chromatographic optimization