三疣梭子蟹精子发生过程中 Pt-DIC 和 Pt-Lamin B 的功能

IF 4.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Molecular Sciences Pub Date : 2023-12-21 DOI:10.3390/ijms25010112

Shuo-Yue Wang, Qiu-Meng Xiang, Junquan Zhu, Chang-Kao Mu, Chun-Lin Wang, Congcong Hou

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引用次数: 0

摘要

细胞质 Dynein 是一种多亚基大分子运动蛋白，参与细胞的运输过程。Dynein中间链（DIC）是Dynein-1的亚基之一。在之前的研究中，我们发现 Pt-DIC 可能在三疣梭子蟹精子形成的核变形过程中扮演重要角色。层粘连蛋白 B 对维持核结构和功能至关重要。令人惊讶的是，在三疣梭子蟹精子形成过程中，Pt-Lamin B不仅在核周围表达，而且还在原顶体中表达。研究还表明，在哺乳动物体内，Dynein-1 可以介导 Lamin B 的运输。因此，为了研究Pt-DIC和Pt-Lamin B在三疣梭子蟹精子发生过程中的关系，我们通过RNAi敲除了三疣梭子蟹的Pt-DIC基因。结果表明，Pt-DIC和Pt-Lamin B在精子发生过程中的分布异常，共定位作用减弱。此外，我们还通过共沉淀验证了Pt-DIC和Pt-Lamin B的相互作用。因此，我们的研究结果表明，Pt-DIC和Pt-Lamin B都参与了三疣梭子蟹的精子发生，而Dynein-1的功能之一就是在三疣梭子蟹的精子发生过程中介导Lamin B的运输。

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The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of Portunus trituberculatus

Cytoplasmic Dynein is a multiple-subunit macromolecular motor protein involved in the transport process of cells. The Dynein intermediate chain (DIC) is one of the subunits of Dynein-1. In our previous studies, we showed that Pt-DIC may play an important role in the nuclear deformation of spermiogenesis in Portunus trituberculatus. Lamin B is essential for maintaining nuclear structure and functions. Surprisingly, Pt-Lamin B was expressed not only in the perinucleus but also in the pro-acrosome during spermiogenesis in P. trituberculatus. Studies have also shown that Dynein-1 can mediate the transport of Lamin B in mammals. Thus, to study the relationship of Pt-DIC and Pt-Lamin B in the spermatogenesis of P. trituberculatus, we knocked down the Pt-DIC gene in P. trituberculatus by RNAi. The results showed that the distribution of Pt-DIC and Pt-Lamin B in spermiogenesis was abnormal, and the colocalization was weakened. Moreover, we verified the interaction of Pt-DIC and Pt-Lamin B via coimmunoprecipitation. Therefore, our results suggested that both Pt-DIC and Pt-Lamin B were involved in the spermatogenesis of P. trituberculatus, and one of the functions of Dynein-1 is to mediate the transport of Lamin B in the spermiogenesis of P. trituberculatus.

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来源期刊

International Journal of Molecular Sciences Chemistry-Organic Chemistry

CiteScore

8.10

自引率

10.70%

发文量

13472

审稿时长

17.49 days

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).