远洋鱼类的眼睛是 IHNV 进入的门户,有助于产生强大的粘膜免疫反应

IF 4.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xinyou Wang, Guangyi Ding, Peng Yang, Gao-feng Cheng, Weiguang Kong, Zhen Xu
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引用次数: 0

摘要

眼粘膜(OM)是脊椎动物粘膜免疫系统的一个重要而独特的组成部分。眼黏膜在维持视觉功能和抵御外来抗原或微生物方面发挥着重要作用,并通过复杂的调节机制保持两者之间的平衡。然而,有关硬骨鱼类眼粘膜对外来病原体的防御功能和粘膜免疫反应的研究仍然较少。为了深入了解远洋鱼类的眼粘膜免疫,我们建立了一项虹鳟(Oncorhynchus mykiss)感染传染性造血坏死病毒(IHNV)后的免疫反应研究。我们的研究结果表明,IHNV 可以成功渗透到虹鳟鱼的组织器官,这表明组织器官可能是 IHNV 的一个重要入口。此外,qPCR和RNA-Seq分析结果表明,在感染IHNV的鳟鱼的OM中,大量免疫相关基因被显著上调。重要的是,我们的 RNA-Seq 分析结果表明,病毒感染引发了强大的免疫反应,这体现在受感染鱼类的组织器官中大量诱导了抗病毒、先天性和适应性免疫相关基因,从而强调了组织器官在病毒感染中的重要作用。总之,我们的研究结果揭示了远缘动物OM在抗病毒防御中的未知功能,为鱼类粘膜免疫研究提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Teleost Eye Is the Portal of IHNV Entry and Contributes to a Robust Mucosal Immune Response
The ocular mucosa (OM) is an important and unique part of the vertebrate mucosal immune system. The OM plays an important role in maintaining visual function and defending against foreign antigens or microorganisms, while maintaining a balance between the two through complex regulatory mechanisms. However, the function of ocular mucosal defense against foreign pathogens and mucosal immune response in bony fish are still less studied. To acquire deeper understanding into the mucosal immunity of the OM in teleost fish, we established a study of the immune response of rainbow trout (Oncorhynchus mykiss) infected with the infectious hematopoietic necrosis virus (IHNV). Our findings revealed that IHNV could successfully infiltrate the trout’s OM, indicating that the OM could be an important portal for the IHNV. Furthermore, qPCR and RNA-Seq analysis results showed that a large number of immune-related genes were significantly upregulated in the OM of trout with IHNV infection. Critically, the results of our RNA-Seq analysis demonstrated that viral infection triggered a robust immune response, as evidenced by the substantial induction of antiviral, innate, and adaptive immune-related genes in the OM of infected fish, which underscored the essential role of the OM in viral infection. Overall, our findings revealed a previously unknown function of teleost OM in antiviral defense, and provided a theoretical basis for the study of the mucosal immunity of fish.
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来源期刊
International Journal of Molecular Sciences
International Journal of Molecular Sciences Chemistry-Organic Chemistry
CiteScore
8.10
自引率
10.70%
发文量
13472
审稿时长
17.49 days
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
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