Evaluation of Biochemical Parameters in Heavy Metals Stressed Crop Phaseolus aconitifolius Cv. RMO 225

Background: Metabolic processes involve set of chemical reactions that occur in the cells of living organisms to sustain life and allow living organisms to maintain their structure and response to the surrounding environment. With increase in industrilisation and urbanisation heavy metal pollution is becoming a global problem. Plant growth and metabolism naturally affected by heavy metals, although they are required in various metabolic reactions and that is why there is need to study their role and impact on biochemical parameters in heavy metal stressed crop. Method: The experiments were carried out during 2019-2020 under laboratory condition. In the present study biochemical constituents of moth (Phaseolus aconitifolius) Jacq. Cv. RMO-225, seedlings were studied under certain heavy metals (Cu, Zn, Pb, Ni and Cd) concentrations 25 ppm-1000 ppm. These concentrations significantly affected biochemical constituents e.g. soluble sugar, starch, phenol and protein content. Result: In the present investigation total sugar and starch content increased at lower concentrations (10-50 ppm concentration) almost in all the treatments except cadmium. However with increase in the concentration (500-100 ppm) total content of both markedly reduced, In cadmium, starch content reduce gradually from 10-1000 ppm concentration. Phenol content also increased at 10-100 ppm concentrations of the heavy metals Cu, Zn, Pb and Ni and decreased at higher concentrations. Protein content sharply decline in all the treatments of cadmium, however in lower concentration of all heavy metals treatments protein content increased and decreased at higher concentrations (200-500 ppm). Cadmium was found to be most toxic in all respect in present study.