初步了解寄生褐青霉中质粒介导的抗性基因的分布和传播情况

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Huarun Sun, Minghui Li, Yilin Bai, Yawei Sun, Chunling Zhu, Xiaojing Xia, Huihui Zhang, Weiyu Luo, Wei Zhang, Yuliang Wen, Yueyu Bai, Lei Wang and Jianhe Hu
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引用次数: 0

摘要

导言。据报道,寄生褐青苔菌中存在多种质粒介导的抗性基因,但人们对其全球分布特征、进化模式和传播方式知之甚少。差距说明。对寄生褐藻疽杆菌中抗性质粒的潜在动员机制研究甚少。研究目的本研究旨在调查寄生虫分离株中质粒介导的抗性基因的普遍性和多样性,重点分析寄生虫抗性质粒的特征。方法。使用 Illumina HiSeq 平台,结合 PCR 和反转 PCR 对检测的质粒进行测序。通过肉汤微稀释法测定宿主菌株的敏感性。测试质粒的转移是通过电穿孔法进行的。使用生物信息学工具比较了本实验室和美国国家生物技术信息中心(NCBI)数据库的序列数据。结果我们实验室鉴定出 19 个质粒,这些抗性质粒具有功能性和可转移性。此外,我们还对寄生虫质粒的五种遗传骨架进行了聚类,并揭示了质粒介导的抗性基因的全球分布特征。结论。这是首次报道在一个寄生虫临床分离株中同时存在携带 tet(H) 的 I 型质粒和携带 lnu(C) 的 II 型质粒。此外,这项研究还首次揭示了质粒介导的抗性基因在全球的分布情况,并根据主干区域对寄生虫中的质粒进行了分类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preliminary view of the distribution and spread of the plasmid-mediated resistance genes in Glaesserella parasuis
Introduction. Various plasmid-mediated resistance genes have been reported in Glaesserella parasuis , but little is known about their global distribution features, evolution pattern and spread. Gap Statement. The potential mobilization mechanisms of resistance plasmids in G. parasuis have been poorly explored. Aim. The aim of the study was to investigate the prevalence and diversity of plasmid-mediated resistance genes among G. parasuis isolates, and focus on the analysis of the features of the resistance plasmids from G. parasuis . Method. The plasmids tested were sequenced using the Illumina HiSeq platform in conjunction with PCR and inverted PCR. The susceptibility of the host strains was determined by broth microdilution. The transfer of plasmids tested was conducted by electroporation. The sequence data were compared using bioinformatics tools and the data from our laboratory and the National Center for Biotechnology Information (NCBI) database. Results. Nineteen plasmids were identified from our laboratory and these resistance plasmids were functional and transferable. Moreover, we clustered five types of genetic backbones of plasmids from G. parasuis and revealed the global distribution features of the plasmid-mediated resistance genes. Conclusions. This is the first report of the coexistence of tet(H)-bearing type I plasmid and lnu(C)-bearing type II plasmid in one G. parasuis clinical isolate. In addition, this study provides the first view of the global distribution of plasmid-mediated resistance genes and classifies the plasmids in G. parasuis according to their backbone regions.
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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