[水溶性非膜蛋白对磷脂结构的影响]。

Biofizika Pub Date : 2002-03-01
G M Sorokoumova, A A Selishcheva, O P Tiurina, S G Alekseeva, O M Martynova, T V Sharf, V I Shvets
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引用次数: 0

摘要

我们利用多胶束囊泡研究了水溶性非膜蛋白质(胰蛋白酶和碱性胰蛋白酶抑制剂(BPTI))与大豆磷脂的相互作用。多胶束囊泡是从大豆脂质提取物和以磷脂酰胆碱为基础的单个磷脂混合物中获得的。这些混合物含有不同的磷脂:"双层"、"非双层 "和带负电荷。研究表明,复合物中两种蛋白质的含量取决于 pH 值和带负电荷成分的存在。根据这一发现,得出了脂质与蛋白质相互作用的静电性质的结论。使用宽线 31P-NMR 光谱法研究了多胶束囊泡中大豆磷脂在蛋白质存在和不存在时的结构组织。研究发现,在成分复杂的磷脂混合物中,不同类型的相共存,不同类别的磷脂可以相互补偿。胰蛋白酶和 BPTI 也以类似的方式影响磷脂的结构,在含有带负电成分的多胶束囊泡中引起相当大的结构变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effect of water soluble non-membrane proteins on the phospholipid structure].

The interaction of water-soluble nonmembraneous proteins (trypsin and the basic pancreatic trypsin inhibitor (BPTI)) with soybean phospholipids was studied using multilamellar vesicles. Multilamellar vesicles were obtained from soybean lipid extracts and mixtures of individual phospholipids based on phosphatidylcholine. These mixtures contain different phospholipids: "bilayer", "nonbilayer", and negatively charged. It was shown that the content of both proteins in the complex depends on pH and the presence of negatively charged components. On the basis of this finding, the conclusion about the electrostatic nature of lipid-protein interaction was made. The structural organization of soybean phospholipids in multilamellar vesicles was studied in the presence and absence of the proteins using broad-line 31P-NMR spectroscopy. It was found that, in mixtures of phospholipids of complex composition, different types of phases coexist, and phospholipids of different classes can compensate the effects of each other. Trypsin and BPTI affect the structure of phospholipids in a similar way, inducing considerable structural changes in multilamellar vesicles of preparations containing negatively charged components in whose structure there coexisted primordially the bilayer and isotropic phases.

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