准确的壳聚糖分光光度法。

IF 2.5 Q3 BIOCHEMICAL RESEARCH METHODS

Biology Methods and Protocols Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI:10.1093/biomethods/bpad036

Oscar González-Davis, Itandehui Betanzo, Rafael Vazquez-Duhalt

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引用次数: 0

摘要

壳聚糖是从甲壳素中提取的一种生物聚合物，甲壳素是自然界中含量最丰富的生物聚合物之一。壳聚糖在生物医学、环境和工业领域的众多应用已广为人知，其潜在应用领域也相当广泛。本研究报告采用了一种新的分光光度法来准确测定壳聚糖的浓度。该方法基于亚硝酸盐在酸性条件下对壳聚糖的脱氨基作用，然后用蒽酮试剂测定碳水化合物。

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An accurate spectrophotometric method for chitosan quantification.

Chitosan is a biopolymer obtained from chitin, one of the most abundant biopolymers in nature. Numerous applications of chitosan are well known in the biomedical, environmental, and industrial fields, and the potential applications are considerable. This work reports a new spectrophotometric method to determine chitosan concentration accurately. The method is based on the deamination of chitosan by nitrite in acidic conditions, followed by a carbohydrate determination by the anthrone reagent.

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来源期刊

Biology Methods and Protocols Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

3.80

自引率

2.80%

发文量

审稿时长

19 weeks