包含mRNA 3 '端cDNA克隆的鉴定

Gene analysis techniques Pub Date : 1989-01-01 DOI:10.1016/0735-0651(89)90009-5

Tom Barry, Marie Stack, Andrew Morgan, Frank Gannon

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引用次数: 3

摘要

提出了一种方法，有利于鉴定cDNA克隆对应的mRNA的聚腺苷化3 '端。它是基于使用一种聚dT探针，这种探针是由市售的低聚dT的均聚延伸而合成的。该方法被证明适用于质粒制备和菌落原位的Southern blot分析。

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The identification of cDNA clones that include the 3′ end of mRNA

A method is presented that facilitates the identification of cDNA clones corresponding to the polyadenylated 3′ end of mRNA. It is based on the use of a poly dT probe that is synthesized by homopolymer extension of commercially available oligo dT. The method is shown to work in Southern blot analysis of plasmid preparations and in situ with colonies.

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Gene analysis techniques

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