Marina Topkoska, Martina Miloshevska, Marjan Piponski, Irena Slaveska Spirevska, Natalija Nakov, Katerina Brezovska, Jelena Acevska
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The greenness of the method was assessed using the analytical eco-scale (AES) methodology and the analytical greenness metric (AGREE). Results The method is selective, linear, precise and accurate over defined concentration ranges (185—369 µg/mL of trans-resveratrol and 37—75 µg/mL of alpha-tocopherol acetate), and has a suitable sensitivity (limits of detection and quantification are 7.7 µg/mL and 23.3 µg/mL for resveratrol and 2.6 µg/mL and 7.8 µg/mL for tocopherol acetate, respectively). The obtained analytical eco-scale score of 77 and the pale green AGREE pictogram with an overall score of 0.61 confirm the method’s greenness. Conclusion The sensitivity and selectivity of the method, its short analysis time (7 minutes), the low negative environmental impact, and simple sample preparation make the method readily applicable to in-line quality control procedures. Highlights A method for simultaneously analysing vitamin E and resveratrol in dietary supplements is presented. 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Determination of the content of both components is essential for confirmation of the quality of the product. Objective To establish the applicability and ensure the greenness of the previously developed high-throughput HPLC/UV method for the simultaneous determination of trans-resveratrol and alpha-tocopherol acetate (vitamin E) in dietary supplements. Methods Separation was performed on RP C8 Select B chromatographic column, using acetonitrile and water in the mobile phase, with gradient elution. Full method validation was performed in accordance with ICH Q2(R1). The greenness of the method was assessed using the analytical eco-scale (AES) methodology and the analytical greenness metric (AGREE). Results The method is selective, linear, precise and accurate over defined concentration ranges (185—369 µg/mL of trans-resveratrol and 37—75 µg/mL of alpha-tocopherol acetate), and has a suitable sensitivity (limits of detection and quantification are 7.7 µg/mL and 23.3 µg/mL for resveratrol and 2.6 µg/mL and 7.8 µg/mL for tocopherol acetate, respectively). The obtained analytical eco-scale score of 77 and the pale green AGREE pictogram with an overall score of 0.61 confirm the method’s greenness. Conclusion The sensitivity and selectivity of the method, its short analysis time (7 minutes), the low negative environmental impact, and simple sample preparation make the method readily applicable to in-line quality control procedures. Highlights A method for simultaneously analysing vitamin E and resveratrol in dietary supplements is presented. 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引用次数: 0
摘要
背景 人们对在膳食补充剂中使用反式白藜芦醇和维生素 E 的组合越来越感兴趣。这两种成分含量的测定对于产品质量的确认至关重要。目的 确定先前开发的同时测定膳食补充剂中反式白藜芦醇和α-生育酚乙酸酯(维生素 E)的高通量 HPLC/UV 方法的适用性并确保其绿色环保性。方法 采用 RP C8 Select B 色谱柱,流动相为乙腈和水,梯度洗脱。根据 ICH Q2(R1)进行了全面的方法验证。采用分析生态尺度(AES)方法和分析绿色度量(AGREE)对该方法的绿色程度进行了评估。结果 该方法在规定的浓度范围内(反式白藜芦醇为 185-369 µg/mL ,α-生育酚醋酸酯为 37-75 µg/mL)具有选择性、线性、精确性和准确性,并具有适当的灵敏度(白藜芦醇的检出限和定量限分别为 7.7 µg/mL 和 23.3 µg/mL,生育酚醋酸酯的检出限和定量限分别为 2.6 µg/mL 和 7.8 µg/mL)。分析生态尺度得分为 77 分,AGREE 图形为淡绿色,总得分为 0.61 分,证实了该方法的绿色环保性。结论 该方法灵敏度高、选择性好、分析时间短(7 分钟)、对环境的负面影响小、样品制备简单,因此可用于在线质量控制程序。亮点介绍了一种同时分析膳食补充剂中维生素 E 和白藜芦醇的方法。该方法快速、样品制备程序简单、对环境影响小。
Greenness assessment and validation of HPLC method for simultaneous determination of Resveratrol and Vitamin E in dietary supplements
Background There is an increasing interest in the use of a combination of trans-resveratrol and vitamin E in dietary supplements. Determination of the content of both components is essential for confirmation of the quality of the product. Objective To establish the applicability and ensure the greenness of the previously developed high-throughput HPLC/UV method for the simultaneous determination of trans-resveratrol and alpha-tocopherol acetate (vitamin E) in dietary supplements. Methods Separation was performed on RP C8 Select B chromatographic column, using acetonitrile and water in the mobile phase, with gradient elution. Full method validation was performed in accordance with ICH Q2(R1). The greenness of the method was assessed using the analytical eco-scale (AES) methodology and the analytical greenness metric (AGREE). Results The method is selective, linear, precise and accurate over defined concentration ranges (185—369 µg/mL of trans-resveratrol and 37—75 µg/mL of alpha-tocopherol acetate), and has a suitable sensitivity (limits of detection and quantification are 7.7 µg/mL and 23.3 µg/mL for resveratrol and 2.6 µg/mL and 7.8 µg/mL for tocopherol acetate, respectively). The obtained analytical eco-scale score of 77 and the pale green AGREE pictogram with an overall score of 0.61 confirm the method’s greenness. Conclusion The sensitivity and selectivity of the method, its short analysis time (7 minutes), the low negative environmental impact, and simple sample preparation make the method readily applicable to in-line quality control procedures. Highlights A method for simultaneously analysing vitamin E and resveratrol in dietary supplements is presented. The method is rapid, includes a simple sample preparation procedure, and has a low environmental impact.
期刊介绍:
The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.