MicroRNA-128通过靶向B淋巴瘤Mo-MLV插入区1抑制胃肠道间质瘤的进展

Jinbao Wu, Changjuan Wang, Xia Cui, Lin Liu, Lu Wang, Jing Wang, Xiaohui Xue, Tong Dang
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引用次数: 0

摘要

简介:microRNA-128(miR-128)在胃肠道相关疾病中的关键作用已被证实。本研究试图阐明 miR-128 在胃肠道间质瘤(GIST)中的特殊作用及其内在机制。然后,对临床组织样本和细胞系中 miR-128 和 B 淋巴瘤 Mo-MLV 插入区 1(BMI-1)的表达模式进行了表征,并验证了它们之间的相关性。结果我们在 78 名患者的 GIST 组织和 4 个 GIST 细胞系中发现了 miR-128 的低表达和 BMI-1 的高表达。异位表达 miR-128 或沉默 BMI-1 可抑制 GIST-T1 细胞的恶性潜能。作为 miR-128 的靶点,BMI-1 的再次表达可以部分抵消 miR-128 对 GIST-T1 细胞恶性程度的抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

MicroRNA-128 acts as a suppressor in the progression of gastrointestinal stromal tumor by targeting B-lymphoma Mo-MLV insertion region 1

MicroRNA-128 acts as a suppressor in the progression of gastrointestinal stromal tumor by targeting B-lymphoma Mo-MLV insertion region 1

Introduction

The critical role of microRNA-128 (miR-128) in gastrointestinal-related diseases has been documented. In the current study, we tried to clarify the specific role miR-128 in gastrointestinal stromal tumor (GIST) and the underlying mechanism.

Methods

Differentially expressed genes in GIST were identified following bioinformatics analysis. Then, expression patterns of miR-128 and B-lymphoma Mo-MLV insertion region 1 (BMI-1) in clinical tissue samples and cell lines were characterized, followed by validation of their correlation. GIST-T1 cells were selected and transfected with different mimic, inhibitor, or siRNA plasmids, after which the biological functions were assayed.

Results

We identified low miR-128 and high BMI-1 expression in GIST tissues of 78 patients and 4 GIST cell lines. Ectopic expression of miR-128 or silencing of BMI-1 suppressed the malignant potentials of GIST-T1 cells. As a target of miR-128, BMI-1 re-expression could partly counteract the suppressive effect of miR-128 on the malignancy of GIST-T1 cells.

Conclusion

Our study provided evidence that miR-128-mediated silencing of BMI-1 could prevent malignant progression of GIST, highlighting a promising anti-tumor target for combating GIST.

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