与还原型谷胱甘肽诱导螅囊排出有关的信号途径

IF 1 4区 生物学 Q3 ZOOLOGY
M. Gavazzi, J. R. Ronderos, M. E. Alzugaray
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引用次数: 0

摘要

在本工作中,我们研究了不同刺激物(活猎物、其匀浆和还原型谷胱甘肽(GSH))对淡水刺胞动物斜水螅(Hydra plagiodesmica, Dioni, 1968)(刺胞动物:水螅)释放信息素的影响。一旦确认了它们的刺激作用,我们选择GSH来分析Ca2+的相关性,感觉受体分子的可能身份,以及驱动放电机制的特定信号通路。我们的研究结果表明,除了机械刺激外,化学刺激本身也可以刺激神经素的释放。使用钙螯合剂,我们验证了由谷胱甘肽触发的放电机制依赖于钙。通过抑制与典型信号通路相关的蛋白质的药物,包括gpcr,我们表明GSH通过l型VGCC引起Ca2+的内流,并通过内质网钙通道释放Ca2+。此外,我们的研究结果表明,GSH的假定受体是GPCR与Gq偶联,其产生IP3和Ca2+作为第二信使。最后,我们发现Hydra vulgaris (Pallas, 1766)的基因组预测了l型VGCC不同亚基的存在,与其他后生动物类群相比,显示出高度的保守性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SIGNALLING PATHWAY ASSOCIATED TO DISCHARGE OF CNIDOCYST INDUCED BY REDUCED GLUTATHIONE IN HYDRA PLAGIODESMICA (DIONI)
In the present work we study the effect of different stimuli (living preys, its homogenate, and reduced glutathione (GSH)) on the discharge of desmonemes in the freshwater cnidarian, Hydra plagiodesmica (Dioni, 1968) (Cnidaria: Hydrozoa). Once confirmed their stimulatory effect, we choose GSH in order to analyse the relevance of Ca2+, the probable identity of the sensory receptor molecule, and the specific signalling pathway driving the discharge mechanism. Our results show that not only mechanical, but also chemical stimuli by itself may stimulate the discharge of desmonemes. Using calcium chelators, we verify that the discharge mechanism triggered by GSH, is dependent on calcium. By mean of drugs that inhibit proteins pertaining to canonical signalling pathways, including GPCRs, we show that GSH causes the influx of Ca2+ via L-type VGCC, and its release by mean of endoplasmic reticulum calcium channels. Moreover, our results suggest that the putative receptor for GSH is a GPCR coupled to Gq, which produces IP3 and Ca2+ as second messengers. Finally, we show that the genome of Hydra vulgaris (Pallas, 1766) predicts the existence of the different subunits of L-type VGCC, showing a high level of conservation when compared with other groups of Metazoa.
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来源期刊
Canadian Journal of Zoology
Canadian Journal of Zoology 生物-动物学
CiteScore
2.40
自引率
0.00%
发文量
82
审稿时长
3 months
期刊介绍: Published since 1929, the Canadian Journal of Zoology is a monthly journal that reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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