Exploring the potential of deep eutectic solvents (DES) in bioactive natural product research: from DES to NaDES, THEDES, and beyond

Deep eutectic solvents (DESs) have garnered significant interest in natural products research, owing to their green and natural attributes in comparison to conventional solvents. However, the approach of demonstrating that DESs are superior extractants has led to an underestimation of their full potential in this field. This perspective disregards crucial challenges related to their practical application and potential scalability, mainly the difficulty of target component separation from intermolecular network forming by DESs. Conversely, the DESs unique features can enhance aspects such as solubilization, stabilization, and storage of natural products, as well as improve their biological activities. By addressing key challenges and limitations, we aim to provide valuable insights into the potential of DESs in this domain. In this review, we conducted an exhaustive literature search to gather relevant articles about DESs and their applications in bioactive natural product research. The gathered literature was analyzed, and a systematic thematic categorization was performed, emphasizing studies where the use of DESs yielded relevant outcomes that could potentially present an advantage in the exploration of bioactive natural products. The approach in structuring this review aimed to provide an overview of the potential and challenges associated with DESs in the domain of bioactive natural product research, transcending their conventional role as mere extraction solvents. Through this critical analysis of the literature, this review delves into the potential of DESs as effective solvents for the solubilization, stabilization, and storage of bioactive natural products. In addition, it highlights the ability of DESs to improve the biological activities of natural products, as well as to be used as formulation media for the transport of pharmaceutical active ingredients. By revealing these advances, the review contributes to a more complete understanding of DESs and its applicability in the field of bioactive natural products research. The studies compiled in this review underscore the expanded potential of DESs, beyond extraction, finding relevance in the realm of natural products research. Notably, they contribute to enhancing the desired attributes of the final product, signifying a promising avenue for future advancements in this field.