用于多色三维共焦旋转盘图像扫描显微镜的可编程综合控制器

IF 2.1 3区工程技术 Q2 MICROSCOPY

Ultramicroscopy Pub Date : 2023-12-10 DOI:10.1016/j.ultramic.2023.113888

Eli Flaxer , Lanna Bram , Alona Flaxer , Yael Roichman , Yuval Ebenstein

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引用次数: 0

摘要

本文为三维共焦旋转盘图像扫描显微镜（CSD-ISM）介绍了一种紧凑、便携、高精度的触发控制系统。在我们之前发表的研究基础上，我们扩展了控制器的硬件，并使其与亚微米级平移器同步，后者可在 Z 方向扫描物体。在扩展硬件的同时，我们还在以前发表的研究成果基础上对软件进行了类似的扩展，使其能够完全控制三维运动。我们展示了由不同高度的二维图像集合而成的清晰流畅的三维图像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Programmable comprehensive controller for multi-color 3D confocal spinning-disk image scanning microscope

This paper introduces a compact, portable, and highly accurate triggering control system for a 3D confocal spinning-disk image scanning microscope (CSD-ISM). Building upon on our previously published research, we expanded the hardware of the controller and synchronized it with a sub-micron translator which scans the object in the z-direction. As well as expanding the hardware, the software also was extended from previously published work similarly as it is stated for hardware while allowing full control over the 3D movement. We showed a clear and smooth 3D image made up of a collection of 2D images at different heights.

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来源期刊

Ultramicroscopy 工程技术-显微镜技术

CiteScore

4.60

自引率

13.60%

发文量

117

审稿时长

5.3 months

期刊介绍： Ultramicroscopy is an established journal that provides a forum for the publication of original research papers, invited reviews and rapid communications. The scope of Ultramicroscopy is to describe advances in instrumentation, methods and theory related to all modes of microscopical imaging, diffraction and spectroscopy in the life and physical sciences.