Camiel Doorenweerd, Michael San Jose, Scott Geib, Norman Barr, Daniel Rubinoff
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We additionally obtained 1,985 Cytochrome C oxidase I (COI) sequences for a subset of the specimens to see which species can be diagnosed with this mtDNA marker and conclude that <i>B. dorsalis</i>, <i>B. incognita</i>, <i>B. carambolae</i> Drew & Hancock, <i>B. raiensis</i> Drew & Hancock, <i>B. occipitalis</i> (Bezzi) and <i>B. kandiensis</i> Drew & Hancock cannot be identified reliably using COI due to introgression—but the newly described species <i>B. borneoensis</i> can be identified using COI. The supposed innocuous species <i>B. raiensis</i> distribution is underestimated in Asia and Africa. <i>Bactrocera kandiensis</i> COI genotypes occur in African flies, but RAD-seq data confirm that these are <i>B. dorsalis</i> with introgressed <i>B. kandiensis</i> COI. The phylogenomic dataset brings new light to the extent of the <i>B. dorsalis s.l.</i> clade and the morphological and molecular confusion based on COI. This will have ramifications for ecological data—including host and distribution ranges—associated with <i>B. dorsalis s.l.</i> clade species, pest identification protocols and our understanding of the economic importance of the various species in the clade.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic data reveal new species and the limits of mtDNA barcode diagnostics to contain a global pest species complex (Diptera: Tephritidae: Dacinae)\",\"authors\":\"Camiel Doorenweerd, Michael San Jose, Scott Geib, Norman Barr, Daniel Rubinoff\",\"doi\":\"10.1111/syen.12616\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The Oriental fruit fly <i>Bactrocera dorsalis</i> (Hendel), a global pest that can decimate regional fruit industries and elicit international quarantines, has been the subject of considerable taxonomic confusion. Previous phylogenetic work revealed that <i>B. dorsalis</i> is part of a monophyletic clade containing 12 species. We present restriction site-associated DNA sequencing (RAD-seq) genomic data for 2,292 specimens, which unequivocally supports the delimitation of two new species, here described as <i>Bactrocera borneoensis</i> <b>sp. n.</b> Doorenweerd & San Jose and <i>B. incognita</i> <b>sp. n.</b> Doorenweerd & San Jose. We additionally obtained 1,985 Cytochrome C oxidase I (COI) sequences for a subset of the specimens to see which species can be diagnosed with this mtDNA marker and conclude that <i>B. dorsalis</i>, <i>B. incognita</i>, <i>B. carambolae</i> Drew & Hancock, <i>B. raiensis</i> Drew & Hancock, <i>B. occipitalis</i> (Bezzi) and <i>B. kandiensis</i> Drew & Hancock cannot be identified reliably using COI due to introgression—but the newly described species <i>B. borneoensis</i> can be identified using COI. The supposed innocuous species <i>B. raiensis</i> distribution is underestimated in Asia and Africa. <i>Bactrocera kandiensis</i> COI genotypes occur in African flies, but RAD-seq data confirm that these are <i>B. dorsalis</i> with introgressed <i>B. kandiensis</i> COI. The phylogenomic dataset brings new light to the extent of the <i>B. dorsalis s.l.</i> clade and the morphological and molecular confusion based on COI. 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引用次数: 0
摘要
东方果蝇背小实蝇(Hendel)是一种全球性害虫,可以摧毁地区水果产业并引发国际隔离,一直是相当大的分类混乱的主题。以前的系统发育工作表明,背孢芽孢杆菌是包含12种的单系分支的一部分。我们提供了2292个标本的限制性内切位点相关DNA测序(RAD-seq)基因组数据,明确支持两个新物种的划分,这里描述为小实蝇(Bactrocera borneensis sp. n. Doorenweerd &圣何塞和B. incognita sp. n. Doorenweerd &圣何塞。我们还获得了1985个细胞色素C氧化酶I (COI)序列,以确定哪些物种可以被诊断为这种mtDNA标记,并得出结论:B. dorsalis, B. incognita, B. carambolae Drew &汉考克,B. raiensis Drew &;汉考克,B. occipitalis (Bezzi)和B. kandiensis Drew &由于基因渐渗,不能用COI可靠地鉴定汉考克,但新描述的B. borneoensis可以用COI鉴定。在亚洲和非洲,被认为是无害物种的白桦的分布被低估了。非洲蝇中存在坎地小实蝇COI基因型,但ad -seq数据证实,这些是带有坎地小实蝇COI基因渐入的背小实蝇。系统基因组学数据的建立使我们对dorsalis s.l的进化分支的范围和基于COI的形态和分子混淆有了新的认识。这将对生态数据产生影响,包括寄主和分布范围,与背salis s.l分支物种,害虫鉴定协议和我们对该分支中各种物种的经济重要性的理解。
Genomic data reveal new species and the limits of mtDNA barcode diagnostics to contain a global pest species complex (Diptera: Tephritidae: Dacinae)
The Oriental fruit fly Bactrocera dorsalis (Hendel), a global pest that can decimate regional fruit industries and elicit international quarantines, has been the subject of considerable taxonomic confusion. Previous phylogenetic work revealed that B. dorsalis is part of a monophyletic clade containing 12 species. We present restriction site-associated DNA sequencing (RAD-seq) genomic data for 2,292 specimens, which unequivocally supports the delimitation of two new species, here described as Bactrocera borneoensissp. n. Doorenweerd & San Jose and B. incognitasp. n. Doorenweerd & San Jose. We additionally obtained 1,985 Cytochrome C oxidase I (COI) sequences for a subset of the specimens to see which species can be diagnosed with this mtDNA marker and conclude that B. dorsalis, B. incognita, B. carambolae Drew & Hancock, B. raiensis Drew & Hancock, B. occipitalis (Bezzi) and B. kandiensis Drew & Hancock cannot be identified reliably using COI due to introgression—but the newly described species B. borneoensis can be identified using COI. The supposed innocuous species B. raiensis distribution is underestimated in Asia and Africa. Bactrocera kandiensis COI genotypes occur in African flies, but RAD-seq data confirm that these are B. dorsalis with introgressed B. kandiensis COI. The phylogenomic dataset brings new light to the extent of the B. dorsalis s.l. clade and the morphological and molecular confusion based on COI. This will have ramifications for ecological data—including host and distribution ranges—associated with B. dorsalis s.l. clade species, pest identification protocols and our understanding of the economic importance of the various species in the clade.
期刊介绍:
Systematic Entomology publishes original papers on insect systematics, phylogenetics and integrative taxonomy, with a preference for general interest papers of broad biological, evolutionary or zoogeographical relevance.