Huda Alrashedi, Sawsan Omer, Adibah Almutairi, Gadah Al-Basher, Osama Mohammed
{"title":"沙特阿拉伯王国Hail地区绵羊、山羊和骆驼奶中黄曲霉毒素M1 (AFM1)的测定及不同方法降低其浓度的评价","authors":"Huda Alrashedi, Sawsan Omer, Adibah Almutairi, Gadah Al-Basher, Osama Mohammed","doi":"10.1080/26395940.2023.2283056","DOIUrl":null,"url":null,"abstract":"ABSTRACT This study aimed to determine aflatoxin M1 (AFM1) in dairy sheep, goats, and camel milk in Hail, Saudi Arabia, and evaluate methods for reducing AFM1. A total of 67 milk samples were collected from Ghazala and Al-Shweimis villages, Hail. AFM1 in milk samples was analyzed using enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) with fluorescence detection. Results revealed concentration of AFM1 in goat and camel milk samples from Ghazala was higher than that in goat and camel milk samples from Al-Shweimis with a significant difference (p<0.05) using both ELISA and HPLC methods. The mean AFM1 concentration using ELISA for goat milk was (12.96 ± 2.23 ng/l), while the AFM1 concentration for camel milk was (1.54 ± 0.29 ng/l). Microwave heating and boiling are effective methods to reduce the AFM1 in milk. The highest AFM1 removal percent was 42.96% by microwave and 12.32 % by boiling milk samples.","PeriodicalId":11785,"journal":{"name":"Environmental Pollutants and Bioavailability","volume":"18 1","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of aflatoxin M1 (AFM1) in dairy sheep, goats and camel milk in Hail Region, Kingdom of Saudi Arabia and evaluation of different methods reducing its concentrations in milk\",\"authors\":\"Huda Alrashedi, Sawsan Omer, Adibah Almutairi, Gadah Al-Basher, Osama Mohammed\",\"doi\":\"10.1080/26395940.2023.2283056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT This study aimed to determine aflatoxin M1 (AFM1) in dairy sheep, goats, and camel milk in Hail, Saudi Arabia, and evaluate methods for reducing AFM1. A total of 67 milk samples were collected from Ghazala and Al-Shweimis villages, Hail. AFM1 in milk samples was analyzed using enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) with fluorescence detection. Results revealed concentration of AFM1 in goat and camel milk samples from Ghazala was higher than that in goat and camel milk samples from Al-Shweimis with a significant difference (p<0.05) using both ELISA and HPLC methods. The mean AFM1 concentration using ELISA for goat milk was (12.96 ± 2.23 ng/l), while the AFM1 concentration for camel milk was (1.54 ± 0.29 ng/l). Microwave heating and boiling are effective methods to reduce the AFM1 in milk. The highest AFM1 removal percent was 42.96% by microwave and 12.32 % by boiling milk samples.\",\"PeriodicalId\":11785,\"journal\":{\"name\":\"Environmental Pollutants and Bioavailability\",\"volume\":\"18 1\",\"pages\":\"\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2023-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Pollutants and Bioavailability\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1080/26395940.2023.2283056\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollutants and Bioavailability","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1080/26395940.2023.2283056","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Determination of aflatoxin M1 (AFM1) in dairy sheep, goats and camel milk in Hail Region, Kingdom of Saudi Arabia and evaluation of different methods reducing its concentrations in milk
ABSTRACT This study aimed to determine aflatoxin M1 (AFM1) in dairy sheep, goats, and camel milk in Hail, Saudi Arabia, and evaluate methods for reducing AFM1. A total of 67 milk samples were collected from Ghazala and Al-Shweimis villages, Hail. AFM1 in milk samples was analyzed using enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) with fluorescence detection. Results revealed concentration of AFM1 in goat and camel milk samples from Ghazala was higher than that in goat and camel milk samples from Al-Shweimis with a significant difference (p<0.05) using both ELISA and HPLC methods. The mean AFM1 concentration using ELISA for goat milk was (12.96 ± 2.23 ng/l), while the AFM1 concentration for camel milk was (1.54 ± 0.29 ng/l). Microwave heating and boiling are effective methods to reduce the AFM1 in milk. The highest AFM1 removal percent was 42.96% by microwave and 12.32 % by boiling milk samples.
期刊介绍:
Environmental Pollutants & Bioavailability is a peer-reviewed open access forum for insights on the chemical aspects of pollutants in the environment and biota, and their impacts on the uptake of the substances by living organisms.
Topics include the occurrence, distribution, transport, transformation, transfer, fate, and effects of environmental pollutants, as well as their impact on living organisms. Substances of interests include heavy metals, persistent organic pollutants, and emerging contaminants, such as engineered nanomaterials, as well as pharmaceuticals and personal-care products as pollutants.