重症COVID-19患者外周血淋巴细胞DNA损伤与炎症标志物和止血参数的关系

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2022-10-26 DOI:10.1093/mutage/geac011
Olgica Mihaljevic,Snezana Zivancevic-Simonovic,Vojislav Cupurdija,Milos Marinkovic,Jovana Tubic Vukajlovic,Aleksandra Markovic,Marijana Stanojevic-Pirkovic,Olivera Milosevic-Djordjevic
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引用次数: 4

摘要

考虑到多种因素可导致基因组不稳定,包括病毒感染,本研究的目的是分析住院COVID-19患者的DNA损伤及其与某些实验室参数的关系。应用治疗和胸部x光对DNA损伤的潜在影响也进行了估计。研究人群包括24例COVID-19重症患者和15例健康对照。以遗传损伤指数(GDI)测定DNA损伤水平。采用标准实验室方法和经认证的酶试剂进行生化和血液学参数的测定。新冠肺炎患者的DNA损伤水平明显高于对照组。与健康对照组相比,新冠肺炎患者中性粒细胞的绝对数量有统计学差异,而淋巴细胞的绝对数量有统计学差异。分析DNA损伤与实验室参数的关系发现,GDI与COVID-19患者白细胞介素6 (IL-6)浓度呈正相关,与血小板计数呈负相关。女性患者的DNA损伤水平略高,GDI与c反应蛋白(CRP)和降钙素原呈正相关。女性人群GDI与血小板计数呈负相关,GDI与活化的部分凝血活素时间(aPTT)呈正相关。应用治疗(抗生素、皮质类固醇、抗凝血剂和抗病毒治疗)以及胸部X射线已被证明具有遗传毒性。DNA损伤水平与COVID-19患者的炎症标志物和止血参数显著对应。总之,炎症、吸烟习惯、应用治疗和胸部X光检查导致COVID-19患者的DNA损伤水平更高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA damage in peripheral blood lymphocytes of severely ill COVID-19 patients in relation to inflammatory markers and parameters of hemostasis.
Bearing in the mind that a variety of agents can contribute to genome instability, including viral infections, the aim of this study was to analyze DNA damage in hospitalized COVID-19 patients and its relationship with certain laboratory parameters. The potential impact of applied therapy and chest X-rays on DNA damage was also estimated. The study population included 24 severely COVID-19 patients and 15 healthy control subjects. The level of DNA damage was measured as genetic damage index (GDI) by comet assay. The standard laboratory methods and certified enzymatic reagents for the appropriate autoanalyzers were performed for the determination of the biochemical and hematological parameters. COVID-19 patients had significantly higher level of DNA damage compared with control subjects. The absolute number of neutrophil leukocytes was statistically higher, while the absolute number of lymphocytes was statistically lower in COVID-19 patients than in healthy controls. The analysis of the relationship between DNA damage and laboratory parameters indicated that GDI was positively correlated with interleukin 6 (IL-6) concentration and negatively with platelet count in COVID-19 patients. The level of DNA damage was slightly higher in female patients, in whom it was demonstrated a positive correlation of GDI with C-reactive protein (CRP) and procalcitonin. Likewise, there was a negative relationship of GDI and platelet count, and positive relationship of GDI and activated partial thromboplastin time (aPTT) in female population. The applied therapy (antibiotics, corticosteroid, anticoagulant, and antiviral therapy) as well as chest X rays has been shown to have genotoxic potential. The level of DNA damage significantly corresponds to the inflammatory markers and parameters of hemostasis in COVID-19 patients. In conclusion, inflammation, smoking habit, applied therapy, and chest X rays contribute to a higher level of DNA damage in COVID-19 patients.
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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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