缺硫条件下莱茵衣藻发酵反应对其连续产氢的影响

Q3 Agricultural and Biological Sciences
A. A. Volgusheva, E. V. Petrova, G. P. Kukarskikh, A. Dubini, T. K. Antal
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引用次数: 3

摘要

摘要研究了在矿物质剥夺条件下,主要发酵酶丙酮酸甲酸裂解酶对绿微藻莱茵衣藻产氢的影响。将无丙酮酸甲酸裂解酶活性的野生型(CC-125)和突变体pfl1在密封的光反应器中无硫培养基上恒定照明培养96小时。在实验过程中,光反应器气相中氢气的积累以及细胞中光合活性、呼吸作用和淀粉含量等影响产氢的因素进行了评估。在完全培养基中培养时,两菌株的光合活性和淀粉含量相似。在硫耗尽条件下,pfl1突变体的培养比野生型更晚开始向氢进化;然而,这个过程在突变体中持续的时间更长。pfl1培养物在硫剥夺初期和后期分别表现出较高的淀粉积累和分解速率。此外,该突变体在剥夺后期具有较高的光合活性。研究结果表明,光合电子传递、氢化酶反应、碳水化合物代谢与发酵过程有着密切的关系,表明利用基因工程修饰发酵反应以提高生物技术中光合产氢的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Influence of Fermentation Reactions on Continuous Hydrogen Photoproduction by Microalga Chlamydomonas reinhardtii under Sulfur Deficiency

Influence of Fermentation Reactions on Continuous Hydrogen Photoproduction by Microalga Chlamydomonas reinhardtii under Sulfur Deficiency

Abstract

The study investigated the effect of the major fermentation enzyme, pyruvate formate lyase, on hydrogen photoproduction by green microalga Chlamydomonas reinhardtii under mineral deprivation. Cultures of the wild type (CC-125) and mutant pfl1 without pyruvate formate lyase activity were incubated on a medium without sulfur in the sealed photoreactors under constant illumination for 96 h. During the experiment, accumulation of hydrogen in a gas phase of photoreactors and factors affecting hydrogen production, such as photosynthetic activity, respiration, and starch content, in cells were assessed. Both strains showed similar photosynthetic activity and starch content when cultivated in the complete medium. Under sulfur depletion, the cultures of the pfl1 mutant began to evolve hydrogen later than the wild type; however, this process lasted much longer in the mutant. The pfl1 cultures showed higher rates of starch accumulation and breakdown during the initial and late stages of sulfur deprivation, respectively. Moreover, the mutant was characterized by higher photosynthetic activity at the later stage of deprivation. The obtained results indicate close relationships between photosynthetic electron transport, hydrogenase reaction, carbohydrate metabolism and fermentation process, pointing to the prospect of using genetic engineering to modify fermentation reactions in order to improve photosynthetic hydrogen production in biotechnology.

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来源期刊
Moscow University Biological Sciences Bulletin
Moscow University Biological Sciences Bulletin Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
0.00%
发文量
18
期刊介绍: Moscow University Biological Sciences Bulletin  is forum for research in all important areas of modern biology. It publishes original work on qualitative, analytical and experimental aspects of research. The scope of articles to be considered includes plant biology, zoology, ecology, evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, gerontology, developmental biology, bioinformatics, bioengineering, virology, and microbiology.
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