I. V. Stonik, R. S. Popov, A. P. Tsurpalo, P. S. Dmitrenok, M. Yu. Shchelkanov, T. Yu. Orlova
{"title":"在H. Peragallo & M. Peragallo, 1900年的假nitzschia H. Peragallo硅藻属培养物中的软骨藻酸,以及来自日本海俄罗斯水域和堪察加半岛太平洋水域的双壳类样品","authors":"I. V. Stonik, R. S. Popov, A. P. Tsurpalo, P. S. Dmitrenok, M. Yu. Shchelkanov, T. Yu. Orlova","doi":"10.1134/s1063074023050115","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The content of a dangerous amnesic toxin, that is, domoic acid (DA), was determined in cultures of the diatom genus <i>Pseudo-nitzschia</i> and in bivalve samples collected in the Russian waters of the Sea of Japan and off the Pacific coast of Kamchatka. For the first time, the presence of DA has been confirmed in cultures of <i>Pseudo-nitzschia pungens</i> (Grunow ex Cleve) G.R. Hasle, 1993 and <i>P. delicatissima</i> (Cleve) Heiden, 1928 from the Pacific coast of Kamchatka with the use of the competitive enzyme-linked immuno-sorbent assay (cELISA). Relatively high concentrations of DA were recorded in the horse mussel <i>Modiolus kurilensis</i> F.R. Bernard, 1983 (2.92 mg/kg based on high-performance chromatography-mass spectrometry (HPLC-MS) and 2.8 mg/kg based on cELISA) and in the Gray mussel <i>Crenomytilus grayanus</i> (0.07 mg/kg based on HPLC-MS and 0.2 mg/kg based on cELISA) collected in the Sea of Japan. It has been shown that for assessing relatively low concentrations of amnesiotoxin (less than 5 ng/mL in microalgal cultures and less than 0.05 mg/kg in mollusks) the cELISA assay is an alternative to HPLC, which does not allow one to detect DA in such samples.</p>","PeriodicalId":49584,"journal":{"name":"Russian Journal of Marine Biology","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Domoic Acid in Cultures of the Diatom Genus Pseudo-nitzschia H. Peragallo in H. Peragallo & M. Peragallo, 1900 and in Bivalve Samples from the Russian Waters of the Sea of Japan and the Pacific Waters of Kamchatka\",\"authors\":\"I. V. Stonik, R. S. Popov, A. P. Tsurpalo, P. S. Dmitrenok, M. Yu. Shchelkanov, T. Yu. Orlova\",\"doi\":\"10.1134/s1063074023050115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>The content of a dangerous amnesic toxin, that is, domoic acid (DA), was determined in cultures of the diatom genus <i>Pseudo-nitzschia</i> and in bivalve samples collected in the Russian waters of the Sea of Japan and off the Pacific coast of Kamchatka. For the first time, the presence of DA has been confirmed in cultures of <i>Pseudo-nitzschia pungens</i> (Grunow ex Cleve) G.R. Hasle, 1993 and <i>P. delicatissima</i> (Cleve) Heiden, 1928 from the Pacific coast of Kamchatka with the use of the competitive enzyme-linked immuno-sorbent assay (cELISA). Relatively high concentrations of DA were recorded in the horse mussel <i>Modiolus kurilensis</i> F.R. Bernard, 1983 (2.92 mg/kg based on high-performance chromatography-mass spectrometry (HPLC-MS) and 2.8 mg/kg based on cELISA) and in the Gray mussel <i>Crenomytilus grayanus</i> (0.07 mg/kg based on HPLC-MS and 0.2 mg/kg based on cELISA) collected in the Sea of Japan. It has been shown that for assessing relatively low concentrations of amnesiotoxin (less than 5 ng/mL in microalgal cultures and less than 0.05 mg/kg in mollusks) the cELISA assay is an alternative to HPLC, which does not allow one to detect DA in such samples.</p>\",\"PeriodicalId\":49584,\"journal\":{\"name\":\"Russian Journal of Marine Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2023-11-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Russian Journal of Marine Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s1063074023050115\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Marine Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1063074023050115","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
Domoic Acid in Cultures of the Diatom Genus Pseudo-nitzschia H. Peragallo in H. Peragallo & M. Peragallo, 1900 and in Bivalve Samples from the Russian Waters of the Sea of Japan and the Pacific Waters of Kamchatka
Abstract
The content of a dangerous amnesic toxin, that is, domoic acid (DA), was determined in cultures of the diatom genus Pseudo-nitzschia and in bivalve samples collected in the Russian waters of the Sea of Japan and off the Pacific coast of Kamchatka. For the first time, the presence of DA has been confirmed in cultures of Pseudo-nitzschia pungens (Grunow ex Cleve) G.R. Hasle, 1993 and P. delicatissima (Cleve) Heiden, 1928 from the Pacific coast of Kamchatka with the use of the competitive enzyme-linked immuno-sorbent assay (cELISA). Relatively high concentrations of DA were recorded in the horse mussel Modiolus kurilensis F.R. Bernard, 1983 (2.92 mg/kg based on high-performance chromatography-mass spectrometry (HPLC-MS) and 2.8 mg/kg based on cELISA) and in the Gray mussel Crenomytilus grayanus (0.07 mg/kg based on HPLC-MS and 0.2 mg/kg based on cELISA) collected in the Sea of Japan. It has been shown that for assessing relatively low concentrations of amnesiotoxin (less than 5 ng/mL in microalgal cultures and less than 0.05 mg/kg in mollusks) the cELISA assay is an alternative to HPLC, which does not allow one to detect DA in such samples.
期刊介绍:
The Russian Journal of Marine Biology was founded in 1975 by Alexey V. Zhirmunsky, member of the Russian Academy of Sciences. The Russian Journal of Marine Biology covers a wide range of research and some applied aspects of marine biology as a synthetic science related to various fields of study on marine biota and environment. It presents fundamental research on biological processes at molecular, cellular, organismal, and populational levels in marine organisms. Consideration is given to marine objects as models in life sciences. The journal also publishes papers dedicated to events in Russian and international marine biological science and the history of biology.