Explanation and prediction for the product of trehalose dihydrate selective dehydration process using mid-frequency Raman difference spectra

Explanation and prediction for the product of trehalose dihydrate dehydration process was realized in this work. β form is the thermodynamic dehydration product of trehalose dihydrate. And α form is the kinetic dehydration product of trehalose dihydrate, which was analyzed by low-frequency Raman spectra, mid-frequency Raman difference spectra and IR difference spectra, and the analysis results confirmed that the crystal structure of trehalose dihydrate and α form are similar. The selective dehydration process from trehalose dihydrate to α form is due to their similar short-range orders. Amorphous trehalose is the uncontrollable dehydration product of trehalose dihydrate due to the collapse of water channels. The dehydration product of trehalose dihydrate by freeze-drying was a mixture of α form and amorphous phase, and the content of amorphous trehalose in the freeze-drying product increases with the decrease of the particle size of dihydrate. Study on the dehydration principle of organic hydrates will guide the preparation, storage and desolvation of drugs and foods.