小麦重复暴露于真菌根部病原体 Bipolaris sorokiniana 会调节根瘤微生物组的组合和病害抑制能力。

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY
Lilian S Abreu Soares Costa, Mírian Rabelo de Faria, Josiane Barros Chiaramonte, Lucas W Mendes, Edis Sepo, Mattias de Hollander, José Maurício Cunha Fernandes, Víctor J Carrión, Wagner Bettiol, Tim H Mauchline, Jos M Raaijmakers, Rodrigo Mendes
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引用次数: 0

摘要

背景:土壤对真菌根部病原体的病害抑制作用通常是通过寄主植物的反复感染以及根瘤微生物组的分类组成和功能特征的相应变化而在田间诱发的。在这里,我们研究了两种小麦栽培品种对 Bipolaris sorokiniana 的这一显著现象,这两种栽培品种对这种真菌根病原体的抗性不同:结果:研究结果表明,易感小麦栽培品种在连续五个生长周期后,病害严重程度显著降低。令人惊讶的是,最初作为对照的抗病小麦栽培品种却表现出相反的模式,在多次接触病原体后病害严重程度增加。扩增子分析表明,细菌科嗜甲壳素科(Chitinophagaceae)、厌氧菌科(Anaerolineaceae)和亚硝基单胞菌科(Nitrosomonadaceae)与易感小麦栽培品种的病害抑制能力有关;抗性小麦栽培品种的病害抑制能力也与嗜甲壳素科(Chitinophagaceae)和较多的拟杆菌科(Comamonadaceae)有关。通过元基因组分析,从 AntiSMASH 分析确定的 2571 个生物合成基因簇(BGC)中筛选出 604 个,这些基因簇在土壤进入抑病状态时具有较高的代表性。这些 BGCs 参与了萜类、非核糖体肽、多酮类、芳基多烯类和翻译后修饰肽的生物合成:结合分类学和功能分析,我们确定了根瘤微生物组在抑制病害过程中的关键变化。这说明了寄主植物是如何依赖根瘤微生物组作为对抗土传病原体的第一道防线的。这里发现的微生物类群和功能可用于控制土传真菌病原体的新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates rhizosphere microbiome assembly and disease suppressiveness.

Background: Disease suppressiveness of soils to fungal root pathogens is typically induced in the field by repeated infections of the host plant and concomitant changes in the taxonomic composition and functional traits of the rhizosphere microbiome. Here, we studied this remarkable phenomenon for Bipolaris sorokiniana in two wheat cultivars differing in resistance to this fungal root pathogen.

Results: The results showed that repeated exposure of the susceptible wheat cultivar to the pathogen led to a significant reduction in disease severity after five successive growth cycles. Surprisingly, the resistant wheat cultivar, initially included as a control, showed the opposite pattern with an increase in disease severity after repeated pathogen exposure. Amplicon analyses revealed that the bacterial families Chitinophagaceae, Anaerolineaceae and Nitrosomonadaceae were associated with disease suppressiveness in the susceptible wheat cultivar; disease suppressiveness in the resistant wheat cultivar was also associated with Chitinophagaceae and a higher abundance of Comamonadaceae. Metagenome analysis led to the selection of 604 Biosynthetic Gene Clusters (BGCs), out of a total of 2,571 identified by AntiSMASH analysis, that were overrepresented when the soil entered the disease suppressive state. These BGCs are involved in the biosynthesis of terpenes, non-ribosomal peptides, polyketides, aryl polyenes and post-translationally modified peptides.

Conclusion: Combining taxonomic and functional profiling we identified key changes in the rhizosphere microbiome during disease suppression. This illustrates how the host plant relies on the rhizosphere microbiome as the first line of defense to fight soil-borne pathogens. Microbial taxa and functions identified here can be used in novel strategies to control soil-borne fungal pathogens.

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来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
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