p元素诱导的睾丸萎缩(PIWI)转录物的表达模式升高是肝细胞癌的潜在候选标志物。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Gehan Hammad, Samah Mamdouh, Dina Mohamed Seoudi, Mohamed Ismail Seleem, Gehan Safwat, Rania Hassan Mohamed
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引用次数: 0

摘要

背景:P-Element-induced wimpy testis (PIWI)蛋白与PIWI相互作用RNA (piRNA)结合,参与生殖细胞基因表达的表观遗传调控。已经发现不同类型的肿瘤细胞表现出piRNA、piwill - mrna和蛋白质的异常表达。我们旨在确定PIWIL1、PIWIL2、PIWIL3和PIWIL4在肝细胞癌患者中的mRNA表达谱,并将其表达模式与临床病理特征联系起来。方法:采用实时定量聚合酶链反应(RT-QPCR)技术检测肝癌患者组织和血清中PIWIL1、PIWIL2、PIWIL3、PIWIL4 mRNA的表达模式,通过ROC曲线评估其对诊断的影响,确定预后效用,并进行预测变异检测、与HCC microrna的相关性和网络分析。结果:HCC组织和血清样本中的表达水平均显著高于各自的对照组(p< 0.001)。此外,对诊断性能进行评估,发现风险测定具有统计学意义。结论:PIWIL mrna在HCC组织和血清样本中过表达,其表达模式与年龄、肿瘤分级和类型相关,可能是HCC有价值的分子标志物。据我们所知,我们的研究首次报道了所有PIWIL mRNA的表达水平,并表明它们作为诊断和预后生物标志物的显著价值,以及它们与HCC发展的相关性。此外,通过DDX4和miR-124-3p对HCC和PIWIL基因进行miRNA预测也可能是一种治疗机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Elevated expression patterns of P-element Induced Wimpy Testis (PIWI) transcripts are potential candidate markers for Hepatocellular Carcinoma.

Background: P-Element-induced wimpy testis (PIWI) proteins, when in combination with PIWI-interacting RNA (piRNA), are engaged in the epigenetic regulation of gene expression in germline cells. Different types of tumour cells have been found to exhibit abnormal expression of piRNA, PIWIL-mRNAs, and proteins. We aimed to determine the mRNA expression profiles of PIWIL1, PIWIL2, PIWIL3, & PIWIL4, in hepatocellular carcinoma patients, and to associate their expression patterns with clinicopathological features.

Methods: The expression patterns of PIWIL1, PIWIL2, PIWIL3, PIWIL4 mRNA, was assessed via real-time quantitative polymerase chain reaction (RT-QPCR), on tissue and serum samples from HCC patients, their impact for diagnosis was evaluated by ROC curves, prognostic utility was determined, and In Silico analysis was conducted for predicted variant detection, association with HCC microRNAs and Network Analysis.

Results: Expression levels were significantly higher in both HCC tissue and serum samples than in their respective controls (p< 0.001). Additionally, the diagnostic performance was assessed, Risk determination was found to be statistically significant.

Conclusion: PIWIL mRNAs are overexpressed in HCC tissue and serum samples, the expression patterns could be valuable molecular markers for HCC, due to their association with age, tumour grade and pattern. To the best of our knowledge, our study is the first to report the expression levels of all PIWIL mRNA and to suggest their remarkable values as diagnostic and prognostic biomarkers, in addition to their correlation to HCC development. Additionally, a therapeutic opportunity might be also suggested through in silico miRNA prediction for HCC and PIWIL genes through DDX4 and miR-124-3p.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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