时间问题:早期和晚期雌激素替代治疗对去卵巢老龄Wistar大鼠葡萄糖代谢和血管反应性的影响。

IF 2.1 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE
Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2023-11-15 eCollection Date: 2023-01-01 DOI:10.1155/2023/6683989
Diana Ramírez-Hernández, Pedro López-Sánchez, Diego Lezama-Martínez, Neidy M Kuyoc-Arroyo, Jessica E Rodríguez-Rodríguez, Salvador Fonseca-Coronado, Ignacio Valencia-Hernández, Jazmin Flores-Monroy
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引用次数: 0

摘要

由于雌激素对心脏保护作用的丧失,绝经后心血管疾病的发病率增加。然而,关于雌激素治疗(ERT)的时机及其对与糖代谢受损相关的血管功能障碍的影响,存在相互矛盾的数据。本研究的目的是评估早期和晚期ERT对老年去卵巢Wistar大鼠血糖/胰岛素平衡和血管反应性的影响。18月龄雌性Wistar大鼠随机分为:(1)假手术,(2)卵巢切除术后10周(10 w),(3)卵巢切除术后10 w +早期雌二醇治疗(10 w-早期E2),(4)卵巢切除术后20周(20 w),(5)卵巢切除术后20周+晚期雌二醇治疗(20 w-晚期E2)。两组均在卵巢切除术后3天给予早期E2, 10周后给予晚期治疗。17β-雌二醇(E2)每日给药(5 μg/kg/d),连续10周。绘制血管紧张素II、KCl和乙酰胆碱(ACh)的浓度-反应曲线。测定心率(HR)、舒张压和收缩压(DBP和SBP)、葡萄糖、胰岛素、HOMA-IR和一氧化氮(NO)水平。除20 w-late E2组外,所有组的血糖水平均高于假手术组。与假手术组相比,所有卵巢切除组胰岛素均升高。除10 w-早期E2组外,所有卵巢切除组的HOMA-IR指数均显示胰岛素抵抗。与10 w组相比,10 w早期E2组一氧化氮水平升高。静脉曲张切除术后10 w,血管对KCl和乙酰胆碱的反应增加,尽管早期给予E2。早期和晚期E2治疗降低了血管对Ang II的反应性。在静脉曲张切除术后20周,舒张压增加,即使使用E2,而收缩压和HR保持不变。E2治疗对血糖/胰岛素平衡和血管反应性的影响取决于治疗的时机。早期ERT可能对胰岛素抵抗和血管功能提供一些保护作用,而晚期ERT可能没有相同的益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Timing Matters: Effects of Early and Late Estrogen Replacement Therapy on Glucose Metabolism and Vascular Reactivity in Ovariectomized Aged Wistar Rats.

Cardiovascular disease incidence increases after menopause due to the loss of estrogen cardioprotective effects. However, there are conflicting data regarding the timing of estrogen therapy (ERT) and its effect on vascular dysfunction associated with impaired glucose metabolism. The aim of this work was to evaluate the effect of early and late ERT on blood glucose/insulin balance and vascular reactivity in aged ovariectomized Wistar rats. Eighteen-month-old female Wistar rats were randomized as follows: (1) sham, (2) 10-week postovariectomy (10 w), (3) 10 w postovariectomy+early estradiol therapy (10 w-early E2), (4) 20-week postovariectomy (20 w), and (5) 20-week postovariectomy+late estradiol therapy (20 w-late E2). Early E2 was administered 3 days after ovariectomy and late therapy after 10 weeks, in both groups. 17β-Estradiol (E2) was administered daily for 10 weeks (5 μg/kg/day). Concentration-response curves to angiotensin II, KCl, and acetylcholine (ACh) were performed. Heart rate (HR), diastolic and systolic blood pressure (DBP and SBP), glucose, insulin, HOMA-IR, and nitric oxide (NO) levels were determined. Higher glucose levels were found in all groups compared to the sham group, except the 20 w-late E2 group. Insulin was increased in all ovariectomized groups compared to sham. The HOMA-IR index showed insulin resistance in all ovariectomized groups, except for the 10 w-early E2 group. The 10 w-early E2 group increased NO levels vs. the 10 w group. After 10 w postovariectomy, the vascular response to KCl and Ach increases, despite early E2 administration. Early and late E2 treatment decreased vascular reactivity to Ang II. At 20-week postovariectomy, DBP increased, even with E2 administration, while SBP and HR remained unchanged. The effects of E2 therapy on blood glucose/insulin balance and vascular reactivity depend on the timing of therapy. Early ERT may provide some protective effects on insulin resistance and vascular function, whereas late ERT may not have the same benefits.

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来源期刊
CiteScore
6.20
自引率
0.00%
发文量
16
审稿时长
6-12 weeks
期刊介绍: JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.
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