中国特有“铁木”红木的基因组组装。

IF 1.9 Q3 GENETICS & HEREDITY
Chang-Yu Wen, Ju-Yu Lian, Wei-Xiong Peng, Zheng-Feng Wang, Zhi-Gang Yang, Hong-Lin Cao
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引用次数: 0

摘要

目的:红麻属是豆科植物中的一个属。该属仅包含约10种,它以其硬木和药用特性而闻名于世。红血球榄。是分布在中国的该属的唯一种。它有优良的木材,可以用于民间医药,这导致了它在野外的过度开发。为了有效地保护和阐明木材形成和医学成分的独特遗传性状,我们提出了它的第一个基因组组装。数据描述:本工作利用紫杉叶组织生成了~ 160.8 Gb的原始纳米孔全基因组测序(WGS)长序列,~ 126.0 Gb的原始MGI WGS短序列和~ 29.0 Gb的原始RNA-seq序列。福地弓形虫基因组de novo片段包含864,825,911 bp,共59个contigs, contig N50为30,830,834 bp。基准测试通用单拷贝正交(BUSCO)显示程序集的完整性为98.7%。该组合包含471,006,885 bp(54.4%)的重复序列和28,761个基因,编码33,803种蛋白质。针对多个数据库对蛋白质序列进行功能注释,便于比较基因组分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome assembly of Erythrophleum Fordii, a special "ironwood" tree in China.

Objectives: Erythrophleum is a genus in the Fabaceae family. The genus contains only about 10 species, and it is best known for its hardwood and medical properties worldwide. Erythrophleum fordii Oliv. is the only species of this genus distributed in China. It has superior wood and can be used in folk medicine, which leads to its overexploitation in the wild. For its effective conservation and elucidation of the distinctive genetic traits of wood formation and medical components, we present its first genome assembly.

Data description: This work generated ~ 160.8 Gb raw Nanopore whole genome sequencing (WGS) long reads, ~ 126.0 Gb raw MGI WGS short reads and ~ 29.0 Gb raw RNA-seq reads using E. fordii leaf tissues. The de novo assembly contained 864,825,911 bp in the E. fordii genome, with 59 contigs and a contig N50 of 30,830,834 bp. Benchmarking Universal Single-Copy Orthologs (BUSCO) revealed 98.7% completeness of the assembly. The assembly contained 471,006,885 bp (54.4%) repetitive sequences and 28,761 genes that coded for 33,803 proteins. The protein sequences were functionally annotated against multiple databases, facilitating comparative genomic analysis.

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