唾液miRNA-184和miRNA-21对口腔鳞状细胞癌和口腔潜在恶性疾病诊断意义的评价

IF 3.2 Q2 PATHOLOGY

Head & Neck Pathology Pub Date : 2023-12-01 Epub Date: 2023-11-28 DOI:10.1007/s12105-023-01600-7

Aarushi Garg, Aadithya B Urs, Bidhan Chandra Koner, Jeyaseelan Augustine, Sameer Ahmad Guru

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引用次数: 0

摘要

背景:miRNAs水平的改变可能影响口腔鳞状细胞癌(OSCC)和口腔潜在恶性疾病(OPMD)的发病机制。本研究评估了唾液miRNA-21和miRNA-184在OSCC和OPMD中的诊断潜力。方法:招募OSCC、OPMD和健康对照共90例。从研究对象的唾液样本中分离出RNA。采用qRT-PCR分析miRNA-21和miRNA-184的表达。比较它们的水平，并使用ROC曲线确定诊断截止值。结果:与健康对照组相比，OSCC和OPMD患者的miRNA-21水平明显升高，miRNA-184水平明显降低(p)。结论:唾液miRNA-21和miRNA-184可能有助于OSCC和OPMD的早期检测。此外，唾液也可用于检测口腔黏膜的肿瘤转化，因为它是无创的，容易获得。

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Evaluation of Diagnostic Significance of Salivary miRNA-184 and miRNA-21 in Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders.

Background: Altered levels of miRNAs might affect the pathogenesis of oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD). This study evaluated the diagnostic potential of salivary miRNA-21 and miRNA-184 in OSCC and OPMD.

Methods: We recruited a total of 90 subjects including OSCC, OPMD, and healthy controls. RNA was isolated from the saliva samples of the study subjects. Expression of miRNA-21 and miRNA-184 was analyzed using qRT-PCR. Their levels were compared and the diagnostic cut-off was determined using the ROC curve.

Results: There was a significant increase in miRNA-21 and a decrease in miRNA-184 in OSCC and OPMD as compared to healthy controls (p < 0.001). Levels of salivary miRNA-21 and miRNA-184 can differentiate OSCC and OPMD from controls and premalignant conditions from malignant conditions.

Conclusion: Salivary miRNA-21 and miRNA-184 may be beneficial for the early detection of OSCC and OPMD. Also, saliva can be used for detecting neoplastic transformation of oral mucosa since it is non-invasive and easily accessible.

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来源期刊

Head & Neck Pathology PATHOLOGY-

CiteScore

5.70

自引率

9.50%

发文量

期刊介绍： Head & Neck Pathology presents scholarly papers, reviews and symposia that cover the spectrum of human surgical pathology within the anatomic zones of the oral cavity, sinonasal tract, larynx, hypopharynx, salivary gland, ear and temporal bone, and neck. The journal publishes rapid developments in new diagnostic criteria, intraoperative consultation, immunohistochemical studies, molecular techniques, genetic analyses, diagnostic aids, experimental pathology, cytology, radiographic imaging, and application of uniform terminology to allow practitioners to continue to maintain and expand their knowledge in the subspecialty of head and neck pathology. Coverage of practical application to daily clinical practice is supported with proceedings and symposia from international societies and academies devoted to this field. Single-blind peer review The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.