脑卒中后对侧海马CA1锥体神经元的结构变化。

IF 5.8 2区 医学 Q1 CLINICAL NEUROLOGY
Brain Pathology Pub Date : 2023-11-27 DOI:10.1111/bpa.13222
Paula Merino-Serrais, Sergio Plaza-Alonso, Farida Hellal, Susana Valero-Freitag, Asta Kastanauskaite, Nikolaus Plesnila, Javier DeFelipe
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引用次数: 0

摘要

在了解中风后成人大脑的反应方面已经取得了重大进展。然而,大量患者在没有适当治疗的情况下继续遭受终身残疾。在本研究中,我们分析了短暂性大脑中动脉闭塞后12-14周缺血性脑卒中小鼠模型tMCAo对侧海马可能的微观解剖改变。将路西法黄单独注射到海马CA1区锥体神经元后,我们对神经元复杂性、树突棘密度和形态进行了详细的三维分析。我们发现,在根尖(辐射层)和基底(东方层),tMCAo小鼠对侧海马CA1锥体神经元的神经元复杂性显著增加,脊柱密度降低,脊柱体积和脊柱长度发生改变。我们的研究结果表明,当同侧海马体严重受损时,对侧海马体表现出几种统计上显著的选择性改变。然而,这些改变并不像预期的那样显著,这可能有助于解释中风后海马功能的恢复。进一步的解剖学和生理学研究是必要的,以更好地了解“完整的”对侧损伤脑区域的改变,这可能是中风后功能恢复的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structural changes of CA1 pyramidal neurons after stroke in the contralesional hippocampus

Structural changes of CA1 pyramidal neurons after stroke in the contralesional hippocampus

Structural changes of CA1 pyramidal neurons after stroke in the contralesional hippocampus

Significant progress has been made with regard to understanding how the adult brain responds after a stroke. However, a large number of patients continue to suffer lifelong disabilities without adequate treatment. In the present study, we have analyzed possible microanatomical alterations in the contralesional hippocampus from the ischemic stroke mouse model tMCAo 12–14 weeks after transient middle cerebral artery occlusion. After individually injecting Lucifer yellow into pyramidal neurons from the CA1 field of the hippocampus, we performed a detailed three-dimensional analysis of the neuronal complexity, dendritic spine density, and morphology. We found that, in both apical (stratum radiatum) and basal (stratum oriens) arbors, CA1 pyramidal neurons in the contralesional hippocampus of tMCAo mice have a significantly higher neuronal complexity, as well as reduced spine density and alterations in spine volume and spine length. Our results show that when the ipsilateral hippocampus is dramatically damaged, the contralesional hippocampus exhibits several statistically significant selective alterations. However, these alterations are not as significant as expected, which may help to explain the recovery of hippocampal function after stroke. Further anatomical and physiological studies are necessary to better understand the modifications in the “intact” contralesional lesioned brain regions, which are probably fundamental to recover functions after stroke.

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来源期刊
Brain Pathology
Brain Pathology 医学-病理学
CiteScore
13.20
自引率
3.10%
发文量
90
审稿时长
6-12 weeks
期刊介绍: Brain Pathology is the journal of choice for biomedical scientists investigating diseases of the nervous system. The official journal of the International Society of Neuropathology, Brain Pathology is a peer-reviewed quarterly publication that includes original research, review articles and symposia focuses on the pathogenesis of neurological disease.
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