S Matuda, K Nakano, T Saheki, R Oyama, M Suzuki, K Titani
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引用次数: 0
摘要
结果表明，大鼠脂酸乙酰转移酶的氨基酸末端序列为(N) ser - leu - pro - his - gln - lys - val - pro - leu - ser - Leu-Ser-Pro-Thr-Met-Gln-Ala-Gly-Thr-Ile-Ala-Arg-Trp-Glu-Lys。此外，还建立了亚基中两个可能的脂酰结合位点的序列，这两个位点彼此非常相似。
Determination of partial amino acid sequence of lipoate acetyltransferase of rat pyruvate dehydrogenase complex.
The partial amino acid sequence of rat lipoate acetyltransferase was determined using the intact protein and the peptides derived from a digest with Achromobacter protease I. The results showed the amino-terminal sequence of the mature enzyme to be (N) Ser-Leu-Pro-Pro-His-Gln-Lys-Val-Pro-Leu-Pro-Ser- Leu-Ser-Pro-Thr-Met-Gln-Ala-Gly-Thr-Ile-Ala-Arg-Trp-Glu-Lys. In addition, the sequences of two possible lipoyl-binding sites in the subunit, which are very similar to each other, were established.
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