与表皮生长因子和钆结合的金纳米颗粒在磁共振成像中用于造影剂的精确递送。

IF 1.7 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Radiological Physics and Technology Pub Date : 2024-03-01 Epub Date: 2023-11-22 DOI:10.1007/s12194-023-00761-y
Suélio M Queiroz, Thaís S Veriato, Leandro Raniero, Maiara L Castilho
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引用次数: 0

摘要

磁共振成像(MRI)造影剂的应用在临床诊断中越来越重要。然而,该技术的低诊断特异性是肿瘤早期发现的限制因素。为了开发一种针对早期肿瘤的新型造影剂,我们合成并表征了一种由金纳米颗粒(AuNPs)、加多巴烯酸(Gd-DTPA)和表皮生长因子(EGF)组成的纳米造影剂。以碳二亚胺为基础的化学修饰Gd-DTPA,使其与aunp功能化。这就形成了Au@Gd-EGF纳米造影剂。利用MRI分析纳米造影剂的松弛率(1/T1),并根据人乳腺腺癌细胞系的细胞活力和线粒体活性测定细胞毒性。傅里叶变换红外光谱分析证实了碳二亚胺在930、1042、1232、1588和1716 cm-1波段存在时对gd - dtpa -半胱胺配合物形成的有效性。这些复合物与aunp表现出良好的相互作用。然而,Au@Gd-EGF纳米造影剂的信号强度低于商用造影剂,因为gd - dtpa基造影剂的r1/r2弛豫度低于gadoversetamide基分子。Au@Gd-EGF纳米造影剂具有良好的生物相容性、低细胞毒性、MRI高信号强度和主动靶向递送,在肿瘤早期诊断中具有重要的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gold nanoparticles conjugated with epidermal growth factor and gadolinium for precision delivery of contrast agents in magnetic resonance imaging.

The utilization of contrast agents in magnetic resonance imaging (MRI) has become increasingly important in clinical diagnosis. However, the low diagnostic specificity of this technique is a limiting factor for the early detection of tumors. To develop a new contrast agent with a specific target for early stage tumors, we present the synthesis and characterization of a nanocontrast composed of gold nanoparticles (AuNPs), gadopentetic acid (Gd-DTPA), and epidermal growth factor (EGF). Carbodiimide-based chemistry was utilized to modify Gd-DTPA for functionalization with AuNPs. This resulted in the formation of the Au@Gd-EGF nanocontrast. The relaxation rate (1/T1) of the nanocontrast was analyzed using MRI, and cytotoxicity was determined based on cell viability and mitochondrial activity in a human breast adenocarcinoma cell line. Fourier-transform infrared spectroscopy analysis confirmed the effectiveness of carbodiimide in the formation of the Gd-DTPA-cysteamine complex in the presence of bands at 930, 1042, 1232, 1588, and 1716 cm-1. The complexes exhibited good interactions with the AuNPs. However, the signal intensity of the Au@Gd-EGF nanocontrast was lower than that of the commercial contrast agent because the r1/r2 relaxivities of the Gd-DTPA-based contrast agents were lower than those of the gadoversetamide-based molecules. The Au@Gd-EGF nanocontrast agent exhibited good biocompatibility, low cytotoxicity, and high signal intensity in MRI with active targeted delivery, suggesting significant potential for future applications in the early diagnosis of tumors.

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来源期刊
Radiological Physics and Technology
Radiological Physics and Technology RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
12.50%
发文量
40
期刊介绍: The purpose of the journal Radiological Physics and Technology is to provide a forum for sharing new knowledge related to research and development in radiological science and technology, including medical physics and radiological technology in diagnostic radiology, nuclear medicine, and radiation therapy among many other radiological disciplines, as well as to contribute to progress and improvement in medical practice and patient health care.
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