检测蛋白-蛋白相互作用的共免疫沉淀试验。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3485-1_16

Mengling Huang, Xiao Yu, Bo Li

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引用次数: 0

摘要

蛋白质通常不是作为单体发挥作用，而是通过与自身或其他蛋白质相互作用来发挥其功能。共免疫沉淀是检测体内蛋白质相互作用的重要方法。在本章中，我们描述了如何使用共免疫沉淀来检测拟南芥原生质体，幼苗和烟叶中的蛋白质相互作用。在使用共免疫沉淀法检测蛋白相互作用时，需要注意实验组和对照组的设计。

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Co-immunoprecipitation Assays to Detect Protein-Protein Interactions.

Proteins usually do not function as monomers but rather perform their functions by interacting with themselves or other proteins. Co-immunoprecipitation is an essential assay for detecting protein interactions in vivo. In this chapter, we describe how to use co-immunoprecipitation to detect protein interactions in Arabidopsis protoplasts, seedlings, and Nicotiana benthamiana leaves. When using co-immunoprecipitation assays to detect protein interactions, it is necessary to pay attention to the design of the experimental and control groups.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.