研究多胎和单胎雌羊卵巢中生育调节lncrna的表达。

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2024-03-01 Epub Date: 2023-11-20 DOI:10.1139/gen-2023-0055
Shahram Hosseinzadeh, Ali Akbar Masoudi
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引用次数: 0

摘要

绵羊是伊朗动物蛋白的主要来源。出生类型是决定肉类总产量的重要特征之一。对于lncrna如何影响产仔数,我们知之甚少。本研究的目的是研究多胎和单胎沙尔羊卵巢组织中的de - lncrna。通过生物信息学分析,发现了母羊之间表达水平不同的lncrna。使用DAVID数据库对目标基因进行注释,并使用STRING和Cytoscape软件评估其相互作用。148个lncrna在多胎和单胎母羊组的表达量差异有统计学意义(FDR < 0.05)。在氧化石墨烯分析中,8个生物过程项、9个细胞成分项、10个分子功能项和38个KEGG通路具有显著性(FDR < 0.05)。影响生育最重要的过程之一是MAPK信号通路,其次是卵母细胞减数分裂、GnRH信号通路、孕激素介导的卵母细胞成熟、催产素信号通路和cAMP信号通路。ENSOARG00000025710、ENSOARG00000025667、ENSOARG00000026034和ENSOARG00000026632是可能影响产仔数和生育能力的lncrna。最重要的枢纽基因包括MAPK1、BRD2、GAK、RAP1B、FGF2、RAP1B和RAP1B。我们希望这项研究能够鼓励研究人员进一步研究lncrna对生育能力的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating the expression of fertility-regulating LncRNAs in multiparous and uniparous Shal ewe's ovaries.

Sheep is the primary source of animal protein in Iran. Birth type is one of the significant features that determine total meat output. Little is known about how long non-coding RNAs (LncRNAs) affect litter size. The purpose of this research is to investigate the DE-LncRNAs in ovarian tissue between multiparous and uniparous Shal ewes. Through bioinformatics analyses, LncRNAs with variable expression levels between ewes were discovered. Target genes were annotated using the DAVID database, and STRING and Cytoscape software were used to evaluate their interactions. The expression levels of 148 LncRNAs were different in the multiparous and uniparous ewe groups (false discovery rate (FDR) < 0.05). Eight biological process terms, nine cellular component terms, 10 molecular function terms, and 38 KEGG pathways were significant (FDR < 0.05) in the GO analysis. One of the most significant processes impacting fertility is mitogen-activated protein kinase (MAPK) signaling pathway, followed by oocyte meiosis, gonadotropin-releasing hormone signaling pathway, progesterone-mediated oocyte maturation, oxytocin signaling pathway, and cAMP signaling pathway. ENSOARG00000025710, ENSOARG00000025667, ENSOARG00000026034, and ENSOARG00000026632 are LncRNAs that may affect litter size and fertility. The most crucial hub genes include MAPK1, BRD2, GAK, RAP1B, FGF2, RAP1B, and RAP1B. We hope that this study will encourage researchers to further investigate the effect of LncRNAs on fertility.

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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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