稀土蛋白复合物的生物学效应:镧系离子Eu3+、Tb3+对钙调素二级结构的影响

Yuan-Yuan Song, Yi-Zhuang Xu, Shi-Fu Weng, Li-Bo Wang, Xiao-Feng Li, Ting-Fang Zhang, Jin-Guang Wu
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引用次数: 0

摘要

四种钙调素的二级结构;通过FTIR分辨率增强技术和曲线拟合,测定了薄膜中芸苔花粉CaM、牛脑CaM、蚯蚓钙结合蛋白和蚯蚓新钙结合蛋白的含量。研究了CaM与Ca2+、Eu3+和Tb3+结合后二级结构的变化、磷酸二酯酶测定和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳。描述了镧系离子对CaM构象的影响。©1999 John Wiley &儿子,Inc。生物光谱学杂志,1999
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biological effects of rare earth protein complexes: Influence of lanthanide ions Eu3+, Tb3+ on secondary structure of calmodulins

The secondary structure of four kinds of calmodulins (CaMs; i.e., Brassica campestris pollen CaM, bovine brain CaM, earthworm calcium binding protein, and earthworm new calcium binding protein) in thin films are determined by the FTIR resolution enhanced technique and curve fitting. The variation in the secondary structure of CaM upon its binding with Ca2+, Eu3+, and Tb3+, the assay of phosphodiesterase enzyme, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis are also investigated. The effect of lanthanide ions on the conformation of CaM are described. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 371–377, 1999

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