{"title":"卟啉的肿瘤定位特性。体外研究使用卟啉前体,氨基乙酰丙酸,在自由和脂质体封装形式。","authors":"H Fukuda, S Paredes, A M Batlle","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Tumor, liver, skin and brain explants from tumor-bearing mice were cultured for 6, 12 and 22 hours in the presence of 0.06, 0.1 and 0.2 mM aminolevulinic acid (ALA). It was found that in all organs, synthesis of porphyrins increased with time and ALA concentration. The synthesising activity of tumor was high, of the same order as that of liver, and nearly twice that of skin and brain. The tissue/tumor porphyrin concentration ratios were lower than 0.5 at longer times and higher ALA concentration. In the case of skin/tumor the lowest ratio was about 0.2 and was obtained with 0.2 mM ALA. Chromatographic analysis of individual porphyrins showed that the whole heme pathway was functional in all organs studied, including tumor. Porphyrin synthesis by different organs from tumor bearing and normal mice was comparatively investigated using free and liposome encapsulated ALA. After 22 hours of incubation with 0.4 mM ALA, porphyrin formation was greater when encapsulated ALA was used. Accumulation of porphyrins in tumor was very high. The levels of activity were the same in each pair of organs in either the tumor-bearing mice or the control. These results indicate that free or encapsulated ALA may be used for the detection of tumors and in photodynamic therapy.</p>","PeriodicalId":11271,"journal":{"name":"Drug design and delivery","volume":"5 2","pages":"133-9"},"PeriodicalIF":0.0000,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tumor-localizing properties of porphyrins. In vitro studies using the porphyrin precursor, aminolevulinic acid, in free and liposome encapsulated forms.\",\"authors\":\"H Fukuda, S Paredes, A M Batlle\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Tumor, liver, skin and brain explants from tumor-bearing mice were cultured for 6, 12 and 22 hours in the presence of 0.06, 0.1 and 0.2 mM aminolevulinic acid (ALA). It was found that in all organs, synthesis of porphyrins increased with time and ALA concentration. The synthesising activity of tumor was high, of the same order as that of liver, and nearly twice that of skin and brain. The tissue/tumor porphyrin concentration ratios were lower than 0.5 at longer times and higher ALA concentration. In the case of skin/tumor the lowest ratio was about 0.2 and was obtained with 0.2 mM ALA. Chromatographic analysis of individual porphyrins showed that the whole heme pathway was functional in all organs studied, including tumor. Porphyrin synthesis by different organs from tumor bearing and normal mice was comparatively investigated using free and liposome encapsulated ALA. After 22 hours of incubation with 0.4 mM ALA, porphyrin formation was greater when encapsulated ALA was used. Accumulation of porphyrins in tumor was very high. The levels of activity were the same in each pair of organs in either the tumor-bearing mice or the control. These results indicate that free or encapsulated ALA may be used for the detection of tumors and in photodynamic therapy.</p>\",\"PeriodicalId\":11271,\"journal\":{\"name\":\"Drug design and delivery\",\"volume\":\"5 2\",\"pages\":\"133-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Drug design and delivery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug design and delivery","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
将荷瘤小鼠的肿瘤、肝脏、皮肤和脑外植体分别在0.06、0.1和0.2 mM氨基乙酰丙酸(ALA)中培养6、12和22小时。结果表明,各器官卟啉的合成随时间和ALA浓度的增加而增加。肿瘤的合成活性很高,与肝脏的合成活性相同,几乎是皮肤和大脑的两倍。时间越长,ALA浓度越高,组织/肿瘤卟啉浓度比均小于0.5。在皮肤/肿瘤的情况下,用0.2 mM ALA获得的比例最低,约为0.2。单个卟啉的色谱分析表明,整个血红素途径在包括肿瘤在内的所有器官中都起作用。用游离ALA和脂质体包膜ALA对荷瘤小鼠和正常小鼠不同脏器合成卟啉进行了比较研究。用0.4 mM ALA孵育22小时后,包封ALA时卟啉的形成更大。肿瘤中卟啉含量高。在荷瘤小鼠和对照组中,每对器官的活性水平是相同的。这些结果表明游离或包封的ALA可用于肿瘤的检测和光动力治疗。
Tumor-localizing properties of porphyrins. In vitro studies using the porphyrin precursor, aminolevulinic acid, in free and liposome encapsulated forms.
Tumor, liver, skin and brain explants from tumor-bearing mice were cultured for 6, 12 and 22 hours in the presence of 0.06, 0.1 and 0.2 mM aminolevulinic acid (ALA). It was found that in all organs, synthesis of porphyrins increased with time and ALA concentration. The synthesising activity of tumor was high, of the same order as that of liver, and nearly twice that of skin and brain. The tissue/tumor porphyrin concentration ratios were lower than 0.5 at longer times and higher ALA concentration. In the case of skin/tumor the lowest ratio was about 0.2 and was obtained with 0.2 mM ALA. Chromatographic analysis of individual porphyrins showed that the whole heme pathway was functional in all organs studied, including tumor. Porphyrin synthesis by different organs from tumor bearing and normal mice was comparatively investigated using free and liposome encapsulated ALA. After 22 hours of incubation with 0.4 mM ALA, porphyrin formation was greater when encapsulated ALA was used. Accumulation of porphyrins in tumor was very high. The levels of activity were the same in each pair of organs in either the tumor-bearing mice or the control. These results indicate that free or encapsulated ALA may be used for the detection of tumors and in photodynamic therapy.