{"title":"使用聚合酶链反应生产离散的高比活性DNA探针","authors":"Ruud Jansen, Fred D. Ledley","doi":"10.1016/0735-0651(89)90020-4","DOIUrl":null,"url":null,"abstract":"<div><p>Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 × 10<sup>9</sup> cpm/μg) is comparable to probes produced by other methods.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"6 4","pages":"Pages 79-83"},"PeriodicalIF":0.0000,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(89)90020-4","citationCount":"44","resultStr":"{\"title\":\"Production of discrete high specific activity DNA probes using the polymerase chain reaction\",\"authors\":\"Ruud Jansen, Fred D. Ledley\",\"doi\":\"10.1016/0735-0651(89)90020-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 × 10<sup>9</sup> cpm/μg) is comparable to probes produced by other methods.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"6 4\",\"pages\":\"Pages 79-83\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(89)90020-4\",\"citationCount\":\"44\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065189900204\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065189900204","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production of discrete high specific activity DNA probes using the polymerase chain reaction
Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 × 109 cpm/μg) is comparable to probes produced by other methods.
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