{"title":"非线性显微镜","authors":"Jerome Mertz","doi":"10.1016/S1296-2147(01)01260-4","DOIUrl":null,"url":null,"abstract":"<div><p>We describe the basic principles of nonlinear optical microscopies based on two-photon excited fluorescence and on second-harmonic generation. Particular attention is given to the physical mechanisms underlying molecular second-harmonic generation, and the features unique to its signal contrast. We provide an overview of some applications of nonlinear microscopy including the visualization of molecular ‘flip–flop’ dynamics in membranes, high-resolution measurements of inter-membrane separations, and high-sensitivity membrane potential imaging. Future developments of nonlinear microscopy are briefly considered.</p></div>","PeriodicalId":100307,"journal":{"name":"Comptes Rendus de l'Académie des Sciences - Series IV - Physics-Astrophysics","volume":"2 8","pages":"Pages 1153-1160"},"PeriodicalIF":0.0000,"publicationDate":"2001-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1296-2147(01)01260-4","citationCount":"0","resultStr":"{\"title\":\"Nonlinear microscopy\",\"authors\":\"Jerome Mertz\",\"doi\":\"10.1016/S1296-2147(01)01260-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We describe the basic principles of nonlinear optical microscopies based on two-photon excited fluorescence and on second-harmonic generation. Particular attention is given to the physical mechanisms underlying molecular second-harmonic generation, and the features unique to its signal contrast. We provide an overview of some applications of nonlinear microscopy including the visualization of molecular ‘flip–flop’ dynamics in membranes, high-resolution measurements of inter-membrane separations, and high-sensitivity membrane potential imaging. Future developments of nonlinear microscopy are briefly considered.</p></div>\",\"PeriodicalId\":100307,\"journal\":{\"name\":\"Comptes Rendus de l'Académie des Sciences - Series IV - Physics-Astrophysics\",\"volume\":\"2 8\",\"pages\":\"Pages 1153-1160\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1296-2147(01)01260-4\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comptes Rendus de l'Académie des Sciences - Series IV - Physics-Astrophysics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1296214701012604\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comptes Rendus de l'Académie des Sciences - Series IV - Physics-Astrophysics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1296214701012604","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
We describe the basic principles of nonlinear optical microscopies based on two-photon excited fluorescence and on second-harmonic generation. Particular attention is given to the physical mechanisms underlying molecular second-harmonic generation, and the features unique to its signal contrast. We provide an overview of some applications of nonlinear microscopy including the visualization of molecular ‘flip–flop’ dynamics in membranes, high-resolution measurements of inter-membrane separations, and high-sensitivity membrane potential imaging. Future developments of nonlinear microscopy are briefly considered.
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