{"title":"应用溴脱氧尿嘧啶免疫组织化学鉴别移植胎儿脑组织。","authors":"D B Brown, B B Stanfield","doi":"10.1155/NP.1989.135","DOIUrl":null,"url":null,"abstract":"<p><p>The immunohistochemical detection of the thymidine analog, 5-bromo-2'-deoxyuridine (BrdU) is shown to be a useful and reliable method to positively identify fetal brain transplants in standard histological preparations. This technique offers several advantages over the [3H]thymidine autoradiographic method, including being much more rapid and avoiding the use of radionuclides.</p>","PeriodicalId":77739,"journal":{"name":"Journal of neural transplantation","volume":"1 3-4","pages":"135-9"},"PeriodicalIF":0.0000,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/NP.1989.135","citationCount":"7","resultStr":"{\"title\":\"The use of bromodeoxyuridine-immunohistochemistry to identify transplanted fetal brain tissue.\",\"authors\":\"D B Brown, B B Stanfield\",\"doi\":\"10.1155/NP.1989.135\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The immunohistochemical detection of the thymidine analog, 5-bromo-2'-deoxyuridine (BrdU) is shown to be a useful and reliable method to positively identify fetal brain transplants in standard histological preparations. This technique offers several advantages over the [3H]thymidine autoradiographic method, including being much more rapid and avoiding the use of radionuclides.</p>\",\"PeriodicalId\":77739,\"journal\":{\"name\":\"Journal of neural transplantation\",\"volume\":\"1 3-4\",\"pages\":\"135-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/NP.1989.135\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of neural transplantation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/NP.1989.135\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of neural transplantation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/NP.1989.135","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The use of bromodeoxyuridine-immunohistochemistry to identify transplanted fetal brain tissue.
The immunohistochemical detection of the thymidine analog, 5-bromo-2'-deoxyuridine (BrdU) is shown to be a useful and reliable method to positively identify fetal brain transplants in standard histological preparations. This technique offers several advantages over the [3H]thymidine autoradiographic method, including being much more rapid and avoiding the use of radionuclides.