Luciano De Petrocellis , Vincenzo Di Marzo , Carmen Gianfrani , Rosario Minei
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引用次数: 11
摘要
1. 研究了水螅体内蛋白激酶C (PKC)激活剂维rucosin B (VB)、1,2- n-二辛烷酰甘油(diC8)和phorol -12-肉豆蔻酸-13-乙酸酯(PMA)对花生四烯酸(AA)及其释放、再摄取和代谢干扰物质的影响。所有PKC激活剂都能有效抑制芽的形成，其中VB和PMA的抑制作用比diC8强10000倍。与水螅共孵育10 min后，VB的作用达到最大，并持续孵育24 h。AA和抑制其从细胞膜再摄取或代谢的物质也能抑制芽的形成，而磷脂酶A2抑制剂油基氧乙基磷胆碱(OOPC)则能有效地诱导芽的形成。本文的研究结果表明，PKC激活和AA都在抑制寻常草芽形成中起作用。
Arachidonic acid, protein kinase C activators and bud formation in Hydra vulgaris
1. The effect of the protein kinase C (PKC) activators verrucosin B (VB), 1,2-sn-dioctanoylglycerol (diC8) and phorbol-12-myristate-13-acetate (PMA), of arachidonic acid (AA) and of substances interfering with its release, re-uptake and metabolism was studied in Hydra vulgaris.
2. All PKC activators potently inhibited bud formation, VB and PMA being 10,000 × more potent than diC8. VB effect was maximal already after 10 min incubation with hydra and persisted at 24 hr incubations.
3. AA and substances inhibiting its re-uptake from cell membrane or its metabolism also inhibited bud formation, whereas oleyl-oxyethyl-phosphorylcholine (OOPC), an inhibitor of phospholipase A2, potently induced bud formation.
4. The findings described herein suggest a role for both PKC activation and AA in the inhibition of bud formation in H. vulgaris.
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