{"title":"羧基荧光素荧光实验","authors":"Stéphane Massou , Renaud Albigot , Michel Prats","doi":"10.1016/S0307-4412(00)00002-9","DOIUrl":null,"url":null,"abstract":"<div><p><span>Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, </span>quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern–Volmer representation for a dynamic quenching.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 171-173"},"PeriodicalIF":0.0000,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00002-9","citationCount":"0","resultStr":"{\"title\":\"Carboxyfluorescein fluorescence experiments\",\"authors\":\"Stéphane Massou , Renaud Albigot , Michel Prats\",\"doi\":\"10.1016/S0307-4412(00)00002-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, </span>quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern–Volmer representation for a dynamic quenching.</p></div>\",\"PeriodicalId\":80258,\"journal\":{\"name\":\"Biochemical education\",\"volume\":\"28 3\",\"pages\":\"Pages 171-173\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00002-9\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical education\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0307441200000029\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical education","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0307441200000029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern–Volmer representation for a dynamic quenching.
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